miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.

By Zissimos Mourelatos, J. Dostie, S. Paushkin, A. Sharma, B. Charroux, L. Abel, Juri Rappsilber, Matthias Mann, G. Dreyfuss. Genes & Development, 2002

1,181 citations recorded by the source corpus.

Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6. Reduction in SMN protein results in Spinal muscular atrophy (SMA), a common neurodegenerative disease. The SMN complex has critical functions in the assembly/restructuring of diverse ribonucleoprotein (RNP) complexes. Here we report that Gemin3 and Gemin4 are also in a separate complex that contains eIF2C2, a member of the Argonaute protein family. This novel complex is a large approximately 15S RNP that contains numerous microRNAs (miRNAs). We describe 40 miRNAs, a few of which are identical to recently described human miRNAs, a class of small endogenous RNAs. The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem-loop structures.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5ea72ccd8c6d68e7d46ae8474be8e75902254a1d

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Citations in the corpus

Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference. (2004, methodology): “As human miRNA loops, m23L and m212L, derived from miR-23 (32,33) and miR-212 (34), respectively, were used.”
Reduced Expression of the let-7 MicroRNAs in Human Lung Cancers in Association with Shortened Postoperative Survival (2004, background): “It has become apparent that genomic information for transcribing miRNAs is indeed implemented in the human genome (6, 9), but extremely little information is available regarding their physiological Table 1 Cox regression analysis of various prognostic factors for postoperative survival of lung cance”
Involvement of microRNA in AU-rich element-mediated mRNA instability. (2005, background): “Two genes located in chromosomes 3 and 13 encode miR16 (Mourelatos et al., 2002).”
Perspective: machines for RNAi. (2005, background): “…et al. 2000, 2002; Aravin et al. 2001, 2003; Lagos-Quintana et al. 2001, 2002, 2003; Lau et al. 2001; Lee and Ambros 2001; Llave et al. 2002a; Mourelatos et al. 2002; Reinhart and Bartel 2002; Brennecke and Cohen 2003; Brennecke et al. 2003; Chen 2004; Chen et al. 2004; Dostie et al. 2003;…”
Human MicroRNA Targets (2004, background): “…Reinhart et al. 2000, 2002; Lagos-Quintana et al. 2001, 2002, 2003; Lau et al. 2001; Lee and Ambros 2001; Llave et al. 2002a; Mette et al. 2002; Mourelatos et al. 2002; Park et al. 2002; Ambros et al. 2003b; Aravin et al. 2003; Brennecke et al. 2003; Dostie et al. 2003; Grad et al. 2003;…”
Clustering and conservation patterns of human microRNAs (2005, background): “However, larger clusters were also identified, such as the human hsa-miR-17 cluster composed of six members (10,11), which is also conserved in other mammals (13), or the Drosophila melanogaster cluster of eight miRNA genes (12).”
A uniform system for microRNA annotation. (2003, background): “MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are found in diverse organisms, including animals (Lagos-Quintana et al. 2001; Lau et al. 2001; Lee and Ambros 2001; Mourelatos et al. 2002) and plants (Llave et al. 2002a; Park et al. 2002; Reinhart et al. 2002).”
SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis. (2004, methodology): “Both of these genes are believed to be involved in transforming ssRNA into the dsRNA precursors of siRNAs (Dalmay et al. 2000; Mourrain et al. 2000).”
The microRNAs of Caenorhabditis elegans. (2003, methodology): “those identified in human cells (Lagos-Quintana et al. 2001; Mourelatos et al. 2002) or are orthologs of miRNAs identified in other”
Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. (2003, background): “Although evidence documenting the structure of premiR-30 has only recently been reported, we note that the most prevalent design for artificial shRNAs also consists of an RNA hairpin bearing a 2-nt overhang (Brummelkamp et al. 2002; Paddison et al. 2002).”