SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis.

By A. Peragine, M. Yoshikawa, Gang Wu, Heidi L. Albrecht, R. Poethig. Genes & Development, 2004

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Higher plants undergo a transition from a juvenile to an adult phase of vegetative development prior to flowering. Screens for mutants that undergo this transition precociously produced alleles of two genes required for posttranscriptional gene silencing (PTGS)--SUPPRESSOR OF GENE SILENCING3 (SGS3) and SUPPRESSOR OF GENE SILENCING2(SGS2)/SILENCING DEFECTIVE1 (SDE1)/RNA-DEPENDENT POLYMERASE6 (RDR6). Loss-of-function mutations in these genes have a phenotype similar to that of mutations in the Argonaute gene ZIPPY (ZIP). Epistasis analysis suggests that ZIP, SGS3, SGS2/SDE1/RDR6, and the putative miRNA export receptor, HASTY (HST), operate in the same pathway(s). Microarray analysis revealed a small number of genes whose mRNA is increased in ZIP, SGS3, and SGS2/SDE1/RDR6 mutants, as well as genes that are up-regulated in SGS3 and SGS2/SDE1/RDR6 mutants, but not in ZIP mutants. One of these latter genes (At5g18040) is silenced posttranscriptionally in trans by the sRNA255 family of endogenous, noncoding, small interfering RNAs (siRNAs). The increase in At5g18040 mRNA in SGS3 and SGS2/SDE1/RDR6 mutants is attributable to the absence of sRNA255-like siRNAs in these mutants. These results demonstrate a role for endogenous siRNAs in the regulation of gene expression, and suggest that PTGS plays a central role in the temporal control of shoot development in plants.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/1222c6a8f696994dd65da5bc39f5d32b9790ce7b

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Citations in the corpus

Uncovering Small RNA-Mediated Responses to Phosphate Deficiency in Arabidopsis by Deep Sequencing1[W][OA] (2009, background): “…to their genomic origin and the interaction of distinct components (Vaucheret, 2006; Vazquez, 2006). ta-siRNAs are phased 21-nucleotide RNA molecules whose synthesis is triggered by the cleavage of miRNA on the TAS transcript (Peragine et al., 2004; Vazquez et al., 2004; Allen et al., 2005).”
microPrimer: the biogenesis and function of microRNA (2005, background): “Arabidopsis plants mutant for ZIPPY (ZIP), an Argonaute gene, and HASTY (HST), which encodes the miRNA export receptor, exhibit a precocious vegetative phenotype and produce abnormal flowers (Peragine et al., 2004).”
microRNA-directed phasing during trans-acting siRNA biogenesis in plants. (2005, background): “RDR6 functions in posttranscriptional RNAi of sense transgenes, some viruses, and specific endogenous mRNAs that are targeted by trans-acting siRNAs (ta-siRNAs) (Dalmay et al., 2000; Mourrain et al., 2000; Parizotto et al., 2004; Peragine et al., 2004; Vazquez et al., 2004b; Yu et al., 2003).”
baySeq: Empirical Bayesian methods for identifying differential expression in sequence count data (2010, background): “It is known that RDR6 is required for production of tasRNAs (trans-acting small RNAs) [21].”
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Micro RNAs and Short‐interfering RNAs in Plants (2007, background): “An initial DCL1-dependent, miRNAguided cleavage of tasiRNA primary transcript defines the 5’ and 3' ends of the transcript which can be converted into dsRNA by RDR6 and SGS3 and sets the 21-nt phase for accurate tasiRNA formation by DCL4 in Arabidopsis (Allen et al. 2005; Gasciolli et al. 2005; Yosh”
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The miRNA156/157 recognition element in the 3' UTR of the Arabidopsis SBP box gene SPL3 prevents early flowering by translational inhibition in seedlings. (2007, background): “Furthermore, a decrease in the steady-state level of SPL3 mRNA could be observed in plants over-expressing the viral suppressor protein P69, negatively affecting the siRNA pathway but promoting the miRNA pathway (Peragine et al., 2004).”
MicroRNA-Directed Regulation of Arabidopsis AUXIN RESPONSE FACTOR17 Is Essential for Proper Development and Modulates Expression of Early Auxin Response Genesw⃞ (2005, background): “, 2004); sgs3 and rdr6 are impaired in the accumulation of trans-acting short interfering RNAs, endogenous small RNAs that, like miRNAs, appear to regulate gene expression by directing mRNA cleavage (Peragine et al., 2004; Vazquez et al., 2004b).”
Biosynthesis and Regulation of Phenylpropanoids in Plants (2017, background): “Ta-siRNAs are a class of endogenous 21nt small interfering RNAs (siRNAs) that can act in trans to repress gene expression through post-transcriptional gene silencing in plants (Peragine et al., 2004; Vazquez et al., 2004; Allen et al., 2005).”
Novel and Mechanical Stress–Responsive MicroRNAs in Populus trichocarpa That Are Absent from Arabidopsisw⃞ (2005, background): “However, the possibility that some of these small RNAs are transacting siRNAs (Parizotto et al., 2004; Peragine et al., 2004; Vazquez et al., 2004b) cannot be ruled out.”
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