Oxidative Stress Sensor Keap1 Functions as an Adaptor for Cul3-Based E3 Ligase To Regulate Proteasomal Degradation of Nrf2

By A. Kobayashi, Moon-Il Kang, H. Okawa, Makiko Ohtsuji, Y. Zenke, T. Chiba, K. Igarashi, Masayuki Yamamoto. Molecular and Cellular Biology, 2004

2,189 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5595beaeab612c49b7aa464c57eabe466148f507

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Citations in the corpus

Oxidative stress response and Nrf2 signaling in aging. (2015, neutral): “Keap1 acts both as an anchor for cytosolic Nrf2 by binding to cytoplasmic actin or myosin VIIa through its DGR domain [349] and as an adaptor of Cul3-based E3 ligase that causes Nrf2 polyubiquitination and proteasomal degradation [30].”
The emerging role of Nrf2 in mitochondrial function (2015, background): “Historically, the ﬁ rst negative regulator of Nrf2 to be discovered was Kelch-like ECH-associated protein 1 (Keap1) [1], a substrate adaptor protein for Cullin 3 (Cul3)/Rbx1 ubiquitin ligase [2 – 4].”
Direct interaction between Nrf2 and p21(Cip1/WAF1) upregulates the Nrf2-mediated antioxidant response. (2009, background): “As a consequence, Nrf2 protein levels are increased, and the Nrf2 signaling pathway is activated (Itoh et al., 1999; Kobayashi et al., 2004, 2006; McMahon et al., 2003; Zhang and Hannink, 2003; Zhang et al., 2004). p21 Cip1/WAF1 regulates many cellular processes such as cellcycle arrest, DNA…”
Nrf2:INrf2(Keap1) Signaling in Oxidative Stress (2009, background): “The main function of INrf2 is to serve as an adapter for the cullin 3/ring box 1 (Cul3/Rbx1) E3 ubiquitin ligase complex [41–43].”
Transcriptional Regulation by Nrf2 (2017, background): “Under unstressed conditions, Nrf2 is constantly targeted for proteasomal degradation by the Keap1/Cul3 E3 ubiquitin ligase complex (37, 60, 80, 192).”
Loss of Keap1 function activates Nrf2 and provides advantages for lung cancer cell growth. (2008, methodology): “Immunoprecipitation, immunofluorescence microscopy, and in vivo ubiquitination assays were performed as described previously ( 7 ).”
Hydrogen peroxide sensing, signaling and regulation of transcription factors (2014, background): “KEAP1 serves as the substrate adaptor subunit in the E3 holoenzyme in the ubiquitination pathway, leading to NRF2 ubiquitination and degradation [186][187].”
Activation of Nrf2-antioxidant signaling attenuates NF-κB-inflammatory response and elicits apoptosis (2008, background): “[4] Kobayashi A, Kang MI, Okawa H, Ohtsuji M, Zenke Y, Chiba T, et al.”
Heme oxygenase-1/carbon monoxide: from basic science to therapeutic applications. (2006, background): “Recent studies suggest that Keap1 recruits cullin3, a subunit of the E3 ligase complex to Nrf2, to promote its ubiquitination (128, 332, 775).”
The Dual Roles of NRF2 in Cancer. (2016, background): “Experiments performed in several models and species have shown that NRF2 activity is negatively regulated by KEAP1, a substrate adapter protein for the CUL3/RBX1 E3 ubiquitin ligase complex [23].”