Multivesicular Endosome Biogenesis in the Absence of ESCRTs

By Susanne Stuffers, Catherine Sem Wegner, Harald A. Stenmark, A. Brech. Traffic : the International Journal of Intracellular Transport, 2009

676 citations recorded by the source corpus.

Subfields: Cell Biology, Epidemiology, Physiology

View Paper: https://www.semanticscholar.org/paper/3dd1a39a71e8248554d0daa0e3b8349254d2c806

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Citations in the corpus

Extracellular Vesicles: Unique Intercellular Delivery Vehicles. (2017, background): “Abbreviations α-SMA α-smooth muscle actin APCs antigen presenting cells CTB cholera toxin B DCs dendritic cells DIC differential interference contrast ECM extracellular matrix ER endoplasmic reticulum ESCRT endosomal sorting complex required for transport EV extracellular vesicle FAK focal adhesion ”
Tetraspanins in Extracellular Vesicle Formation and Function (2014, background): “Interestingly, in the absence of ESCRTs, MVBs, and exosomes may be formed, so that cells in which the four subunits of the ESCRT complex have been depleted are able to generate CD63-positive EVs (72).”
Biogenesis and secretion of exosomes. (2014, neutral): “Of note, mammalian cells depleted for key ESCRT components still form MVBs [45].”
Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake (2016, neutral): “For example, inactivation of the ESCRTs does not inhibit the formation of MVBs (Stuffers et al. 2009).”
Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression (2018, background): “Multivesicular bodies and EXOs can also form in the absence of ESCRT machinery (54), and tetraspanins seem to be particularly important in this ESCRT-independent mechanism of ILV and EXO biogenesis.”
Exosomes and other extracellular vesicles in host–pathogen interactions (2014, positive): “Proteomic analysis of exosomes has demonstrated the presence of ESCRT machinery within exosomes, and knockdown of key components of ESCRT machinery can abrogate ILV formation and exosome release [39], although this is likely cell type-specific [40,41].”
Current knowledge on exosome biogenesis and release (2017, background): “For example, it has been shown that despite simultaneously silencing of key subunits of all four ESCRT-complexes, ILVs are still formed in MVBs, thus indicating the presence of ESCRT-independent mechanisms [38].”
Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles (2013, background): “This fully corroborates the observations that cells contain different MVBs and that within MVBs, ILVs are not all similar in morphology and composition (Fevrier et al., 2004; Möbius et al., 2002; White et al., 2006).”
Syndecan–syntenin–ALIX regulates the biogenesis of exosomes (2012, background): “Note that truncated forms of Alix containing the V domain interact with the two parts of the N-Ter domain of syntenin that contain LYPXL motifs, (1-30) and (31-60), and that mutation of the three LYPXL motifs (YP to AA) abolishes the interaction.”
Exosomes--vesicular carriers for intercellular communication. (2009, background): “Note added in proof The papers refered to in the text as H Stenmark and W Stoorvogel personal communication have now been accepted for publication [65,66].”
Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, background): “Abbreviation: PM, plasma membrane. does not abolish MVB formation (Stuffers et al. 2009).”
Endosome maturation (2011, background): “Inhibition of VPS34 activity causes enlarged LEs with fewer ILVs, and EGFR accumulates in the limiting membrane (Futter et al, 2001).”
The tetraspanin CD63 regulates ESCRT-independent and dependent endosomal sorting during melanogenesis (2011, background): “…of the TSPAN family were predicted to participate in ESCRT-independent ILV sorting because they are highly enriched in ILVs, even in the absence of functional ESCRT components (Buschow et al., 2009; Simons and Raposo, 2009; Stuffers et al., 2009; Trajkovic et al., 2008; van Niel et al., 2006).”
SORTING IT OUT: REGULATION OF EXOSOME LOADING (2014, background): “Accordingly, MVBs are still present when Hrs, tsg101, Vsp22 and Vps24 are simultaneously silenced in mammals, whereas EGF-induced MVB formation is impaired [28].”
Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes (2019, background): “[46] demonstrat d that MVB bi genesis can occur without ESCRTs.”