Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles

By Marina Colombo, Catarina F Moita, Guillaume van Niel, J. Kowal, James Vigneron, P. Benaroch, N. Manel, L. Moita, C. Théry, G. Raposo. Journal of Cell Science, 2013

1,405 citations recorded by the source corpus.

Subfields: Immunology, Immunology and Allergy, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/618a5613b5676c7575055f7fe62d49a4f29aa2be

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Citations in the corpus

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes (2016, methodology): “Heterogeneity in size of EVs recovered by DUC from these cells (13), and the presence of membrane-enclosed structures at the DC surface (Fig.”
Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, methodology): “…some common mechanisms (decrease of exosome secretion by TSG101 depletion) but also discrepancies: inhibition (Baietti et al. 2012) versus increase (Colombo et al. 2013) of exosome secretion induced by VPS4B depletion and inhibition of exosome release by ALIX depletion in MCF7 and possibly in DCs…”
SORTING IT OUT: REGULATION OF EXOSOME LOADING (2014, background): “A recent study shows the effect of silencing twenty-three components of the ESCRT machinery by RNAi in MHC-II-expressing HeLa cells [21].”
Tetraspanins in Extracellular Vesicle Formation and Function (2014, background): “A recent study using RNA interference (RNAi) to target 23 different components of the ESCRT machinery and associated proteins, highlights the cell-specificity of the mechanism of protein sorting and secretion in exosomes (65).”
Lipids in exosomes: Current knowledge and the way forward. (2017, background): “However, removal or inhibition of nSMase do not affect exosome release in all cell lines tested [77-79].”
Extracellular Vesicle Heterogeneity: Subpopulations, Isolation Techniques, and Diverse Functions in Cancer Progression (2018, methodology): “evaluated effects on secretion of EXOs (100,000 × g pellets) by HeLa-CIITA-OVA and dendritic cells (DCs) (53).”
Challenges and directions in studying cell–cell communication by extracellular vesicles (2022, background): “This occurs most likely once EVs have been internalized, although direct fusion of EVs with the recipient cell has also been suggested (8).”
Exosomes: key players in cancer and potential therapeutic strategy (2020, background): “The components of ESCRTs (containing ~20 proteins) comprises four complexes, termed ESCRT-0, -I, -II, and -III, with associated proteins, including ALIX (Apoptosis-linked gene 2-interacting protein X, encoded by PDCD6IP), VTA1(Vesicle Trafficking 1), VPS4 (Vacuolar protein sorting-associated protein”