TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm.

By A. Vassilev, Kotaro J Kaneko, Hongjun Shu, Y. Zhao, M. DePamphilis. Genes & Development, 2001

744 citations recorded by the source corpus.

Mammals express four highly conserved TEAD/TEF transcription factors that bind the same DNA sequence, but serve different functions during development. TEAD-2/TEF-4 protein purified from mouse cells was associated predominantly with a novel TEAD-binding domain at the amino terminus of YAP65, a powerful transcriptional coactivator. YAP65 interacted specifically with the carboxyl terminus of all four TEAD proteins. Both this interaction and sequence-specific DNA binding by TEAD were required for transcriptional activation in mouse cells. Expression of YAP in lymphocytic cells that normally do not support TEAD-dependent transcription (e.g., MPC11) resulted in up to 300-fold induction of TEAD activity. Conversely, TEAD overexpression squelched YAP activity. Therefore, the carboxy-terminal acidic activation domain in YAP is the transcriptional activation domain for TEAD transcription factors. However, whereas TEAD was concentrated in the nucleus, excess YAP65 accumulated in the cytoplasm as a complex with the cytoplasmic localization protein, 14-3-3. Because TEAD-dependent transcription was limited by YAP65, and YAP65 also binds Src/Yes protein tyrosine kinases, we propose that YAP65 regulates TEAD-dependent transcription in response to mitogenic signals.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/339dab01b83c8b02b366ee3261c4ebc313a958fd

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In Cell Biology

1. K. Guan
2. Bin Zhao
3. G. Halder
4. Duojia Pan
5. Fa-Xing Yu
6. S. Piccolo
7. Randy L. Johnson
8. M. Cordenonsi

In Molecular Biology

1. K. Guan
2. Duojia Pan
3. Bin Zhao
4. G. Halder
5. Randy L. Johnson
6. Fa-Xing Yu
7. S. Piccolo
8. M. Cordenonsi

Citations in the corpus

• Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse (2009, background): “However, molecules such as Lgl (lethal giant larva homolog) and the PAR polarity protein Par1 are localized exclusively in the basolateral regions of each blastomere (Vinot et al., 2005) (Fig.”
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• The Hippo Signaling Pathway in Development and Disease (2019, background): “Not only were they identified as Yki/YAP-binding proteins in multiple unbiased protein-protein interaction screens in both Drosophila and mammals (Vassilev et al., 2001; Wu et al., 2008), they were also shown to bind to various Hippo target genes such as diap1 (Koontz et al.”
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• The TEAD/TEF family of transcription factor Scalloped mediates Hippo signaling in organ size control. (2008, background): “The mammalian Yki homolog, YAP, can function as a coactivator for several transcription factors including the Runt family member PEBP2α, the P53 family member P73, and the TEAD/TEF family of transcription factors (Strano et al., 2001; Vassilev et al., 2001; Yagi et al., 1999).”
• Hippo signaling in organ size control. (2007, background): “YAP, the mammalian homolog of Yki, has been reported to function as the coactivator for several transcription factors, such as the p53 family member p73, the Runt family member PEBP2 , and the four TEAD/TEF transcription factors (Yagi et al. 1999; Strano et al. 2001; Vassilev et al. 2001).”
• TEAD mediates YAP-dependent gene induction and growth control. (2008, background): “Several transcription factors, including ErbB4, Runx2, TEAD, and p73, have been reported to interact with YAP (Yagi et al. 1999; Vassilev et al. 2001; Basu et al. 2003; Komuro et al. 2003).”
• The Hippo Signaling Pathway in Development and Cancer (2010, background): “Studies in mammalian cells have revealed multiple DNA-binding transcription factor partners for YAP, such as the p53 family member p73 (Strano et al., 2001), the Runt family member Runx2 (Yagi et al., 1999), and the TEAD/TEF family transcription factors (Vassilev et al., 2001).”
• The biology of YAP/TAZ: hippo signaling and beyond. (2014, background): “TEADs have been repeatedly isolated as main YAP and TAZ interacting transcription factors in mass-spectrometry experiments (29, 209, 252), in line with results in Drosophila, in which the TEAD homolog Scalloped was identified as key (although not exclusive) partner for Yorkie transcriptional activit”
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• Elucidation of a universal size-control mechanism in Drosophila and mammals. (2007, background): “…cells, including EBP50 (Mohler et al., 1999), Smad7 (Ferrigno et al., 2002), ErbB4 (Komuro et al., 2003), p53BP-2 (Espanel and Sudol, 2001), p73 (Basu et al., 2003), and hnRNAP U (Howell et al., 2004), as well as Runt (Yagi et al., 1999) and TEAD (Vassilev et al., 2001) transcription factors.”
• The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. (2009, background): “Tead proteins are known to act in conjunction with the coactivator protein Yap (Vassilev et al., 2001), whose nuclear localization is regulated by phosphorylation (Zhao et al., 2007).”
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• The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway. (2008, background): “the TEAD/TEF family transcription factors (Vassilev et al., 2001; Giot et al., 2003).”
• Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. (2007, background): “YAP has been shown to interact with and activate the TEAD family transcription factors, which have four highly conserved members (Vassilev et al. 2001).”
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