An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus.

By T. Dalmay, A. Hamilton, S. Rudd, S. Angell, David C. Baulcombe. Cell, 2000

1,117 citations recorded by the source corpus.

Subfields: Endocrinology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/1fd24d6aee1e4c624fb3ce59e9af2a59a9b7442c

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Citations in the corpus

Gardening the genome: DNA methylation in Arabidopsis thaliana (2005, background): “Mutations in the RNA-DEPENDENT RNA POLYMERASE 6 ( RDR6 ), SUPPRESSOR OF GENE SILENCING 3/SILENCING DEFECTIVE 2 ( SGS3/SDE2 ) and SILENCING DEFECTIVE 3 ( SDE3 ) genes impair virus-induced gene silencing and also cause defects in the RNAi-like silencing that is induced by highly transcribed sense ”
siRNA function in RNAi: a chemical modification analysis. (2003, background): “A second factor in amplification is the presence of RNA-dependent RNA polymerase (RdRP), which has been found in plants, worms, fungi, and flies (Cogoni and Macino 1999; Dalmay et al. 2000; Sijen et al. 2001).”
Fertile Hypomorphic ARGONAUTE (ago1) Mutants Impaired in Post-Transcriptional Gene Silencing and Virus Resistance (2002, background): “Because all other Arabidopsis PTGS-deficient mutants ( sgs1 to sgs3 and sde1 to sde4 ) (Elmayan et al., 1998; Dalmay et al., 2000; Mourrain et al., 2000) show no significant defect in development, the possible link between PTGS and development in plants is unclear.”
Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis (2004, background): “Because TIR1 is a positive regulator of auxin signaling by promoting the degradation of Aux/IAA proteins through ubiquitination (Dharmasiri and Estelle, 2002), the miRNA inhibition of TIR1 would downregulate auxin signaling and seedling growth.”
A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis. (2005, methodology): “…pathway is unlikely to be restricted to this class of transcripts because two of its components, SGS3 and RDR6, are required for PTGS of sense transgenes and for resistance to some viruses (Dalmay et al. 2000b; Mourrain et al. 2000; Beclin et al. 2002; Himber et al. 2003; Muangsan et al. 2004).”
Genetic and Functional Diversification of Small RNA Pathways in Plants (2004, background): “Arabidopsis plants contain at least three active RdRp genes, termed RDR1, RDR2, and RDR6 (also known as SDE1/SGS2) (Dalmay et al. 2000; Mourrain et al. 2000; Yu et al. 2003).”
RNA interference is mediated by 21- and 22-nucleotide RNAs. (2001, background): “Genetic screens in C. elegans identified rde-1 and rde-4 as essential for activation of RNAi, without an effect on transposon mobilization or cosuppression (Tabara et al. 1999; Dernburg et al. 2000; Grishok et al. 2000; Ketting and Plasterk 2000).”
Approaches to Minimize Variation of Transgene Expression in Plants (2005, background): “…genes presumed to play a role in the PTGS-machinery, including genes encoding a putative RNA-dependent RNA polymerase (RdRp) (SGS2/SDE1/ RDR6; Dalmay et al. 2000; Mourrain et al. 2000; Xie et al. 2004), a coiled-coil protein of unknown function (SGS3; Mourrain et al. 2000), a RNA helicase…”
Small RNAs in transcriptional gene silencing and genome defence (2009, background): “These RNAs recruit RNA-dependent RNA polymerase (RdRP) enzymes, which recognize free 3′ ends and synthesize dsRN”
On the role of RNA amplification in dsRNA-triggered gene silencing. (2001, neutral): “…for a measurable re- RNA-triggered silencing by a variety of sense transgenes and for RNA-triggered defense against some butsponse (perhaps incorporation of injected siRNAs into RISC complexes [Hammond et al., 2001] is much less not all viral genomes (Mourrain et al., 2000; Dalmay et al., 2000).”