On the role of RNA amplification in dsRNA-triggered gene silencing.

By T. Sijen, J. Fleenor, F. Simmer, K. Thijssen, S. Parrish, L. Timmons, Ronald H. A. Plasterk, Andrew Fire. Cell, 2001

1,380 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/2735c58157668ebbd14a2705a4802bf5a42b9c78

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In Molecular Biology

Citations in the corpus

Novel and Stress-Regulated MicroRNAs and Other Small RNAs from Arabidopsis (2004, background): “…of host- or viral-encoded RNAdependent RNA polymerases (Dalmay et al., 2000; Mourrain et al., 2000; Aravin et al., 2001; Grishok et al., 2001; Sijen et al., 2001). siRNAs assemble in complexes termed RNA-induced silencing complexes and target homologous RNA sequences for endonucleolytic…”
Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. (2002, neutral): “An independently isolated transposon insertion In addition to the effects on RNAi, we have also oballele (pk2042) [4] displays identical phenotypes.”
Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. (2010, background): “Also in nematodes, RdRp orthologs were found (Sijen et al., 2001; Tomoyasu et al., 2008).”
Circular RNA Is Expressed across the Eukaryotic Tree of Life (2014, neutral): “, [8,15,16]), and reasoned that even sequence datasets from poly(A)-selected RNA could provide evidence of circular RNA expression even if not for quantitative estimates of abundance.”
Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. (2007, neutral): “In C. elegans, effective RNAi depends upon an amplification mechanism (Sijen et al., 2001).”
Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans (2003, methodology): “This was true also when we used a mutant strain with increased RNAi sensitivit”
A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans (2004, background): “By contrast, eri-1 enhanced sensitivity to hmr-1 and dpy-13 dsRNAs in an rrf-1 -null mutant background, suggesting that amplification of secondary siRNAs in somatic tissues is not essential for eri-1 to enhance RNAi (ref”
Endogenous and Silencing-Associated Small RNAs in Plants Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003210. (2002, neutral): “elegans and Drosophila (Lipardi et al., 2001; Sijen et al., 2001).”
Recent advances in RNA interference research in insects: Implications for future insect pest management strategies (2013, background): “the front end of the RNAi pathway (Sijen et al., 2001).”
Insulin signaling promotes germline proliferation in C. elegans (2010, methodology): “To investigate further the germline versus soma activity of pathway components, we tested the efficacy of RNAi knockdown of several pathway components in the rrf-1 mutant background that reduces RNAi effectiveness in the soma but retains it the germ line [rrf1(pk1417) (Sijen et al., 2001)].”
Perspective: machines for RNAi. (2005, background): “In C. elegans, dsRNA is thought to first be diced into rare primary siRNAs and then amplified by RdRP enzymes to create more abundant secondary siRNAs (Sijen et al. 2001).”
siRNA function in RNAi: a chemical modification analysis. (2003, background): “RdRP has been postulated to amplify target mRNA, through a random, degradative PCR model (Lipardi et al. 2001; Nishikura 2001; Sijen et al. 2001), into dsRNA, which can be targeted by Dicer.”
Induction and suppression of RNA silencing: insights from viral infections (2005, background): “1 ), which have been identified in plants, Caenorhabditis elegans and fungi, but not in insects or vertebrate”