Efficient genome editing in plants using a CRISPR/Cas system

By Zhengyan Feng, Botao Zhang, W. Ding, Xiaodong Liu, Dong-Lei Yang, Peng-lian Wei, Fengqiu Cao, Shihua Zhu, Feng Zhang, Yanfei Mao and 1 more. Cell Research, 2013

972 citations recorded by the source corpus.

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Subfields: Business and International Management, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f167402ab3d5deaf6df1373f81967800c124bfc0

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Citations in the corpus

The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “In August 2013, five reports were published discussing the first application of CRISPR/Cas9-based genome editing in plants (Feng et al., 2013; Li et al., 2013; Nekrasov et al., 2013; Shan et al., 2013; Xie and Yang, 2013).”
The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation. (2014, methodology): “, 2013) and recently applied to gene modification in plants (Feng et al., 2013; Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Miao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013).”
Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology. (2016, background): “Off-target cleavage of Cas9/sgRNA occurs in mammalian and plant cells, and is more frequent if the first 12 nucleotides linked to the NGG sequence (PAM) are identical to the target sequence (Fu et al., 2013; Zhang et al., 2014).”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “The CRISPR/Cas system has been harnessed to achieve efficient genome editing in a variety of organisms, including bacteria, yeast, plants, and animals, as well as human cell lines [12-27].”
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew (2014, neutral): “This phenomenon has also been reported in diploid Arabidopsis 14 and rice 25 .”
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, methodology): “, 2014), human codon-optimized 196 Cas9 (hSpCas9, pYPQ158) (Feng et al., 2013; Mao et al., 2013; Feng et al., 2014; Zhang et al., 197 2014) and a plant codon-optimized Cas9 harboring enriched GC content within key 5’ coding 198”
Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922 (2016, background): “This phenomenon has been reported in rice [29, 34, 35, 38], Arabidopsis [74], wheat [47], tomato [49] and maize [42].”
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “However, transgenic lines expressing CRISPR/Cas9 have mainly been mosaic in the first generation (T1), indicating that CRISPR/Cas9-induced mutations in Arabidopsis occurred after the first embryonic cell division [20, 22, 25, 26, 29, 35].”
Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis (2014, background): “Materials and Methods Detailed information is provided in SI Appendix and our previous publications (6, 7).”
Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN] (2015, background): “Testing of S. pyogenes Cas9-gRNA in a variety of plant species has resulted in extremely low frequency of nonspecific DNA cleavage and therefore, high specificity of the system (Feng et al., 2013; Shan et al., 2013; Brooks et al., 2014; Zhang et al., 2014).”