Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

By Yanpeng Wang, Xi Cheng, Qi-wei Shan, Yi Zhang, Jinxing Liu, Caixia Gao, J. Qiu. Nature Biotechnology, 2014

1,748 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/c0c8ea208a61f00ecec5908084e0162435462030

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Caixia Gao
2. M. Meissle
3. Jörg Romeis
4. S. Naranjo
5. A. Shelton
6. Z. Lippman
7. Kunling Chen
8. Yanpeng Wang

In Plant Science

1. Caixia Gao
2. Jörg Romeis
3. M. Meissle
4. S. Naranjo
5. A. Shelton
6. Z. Lippman
7. Kunling Chen
8. Yanpeng Wang

Citations in the corpus

• Heat and Drought Stresses in Crops and Approaches for Their Mitigation (2018, methodology): “Wang et al. (2014) used TALEN and CRISPR-Cas9 technologies in hexaploid bread wheat to introduce targeted mutations in the three homoeoalleles that encode MILDEW-RESISTANCE LOCUS (MLO) proteins.”
• Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System1 (2014, background): “…Arabidopsis (Arabidopsis thaliana; Li et al., 2013) and Nicotiana benthamiana (Nekrasov et al., 2013), there have also been reports for three crop species, rice (Oryza sativa; Zhang et al., 2014), sorghum (Sorghum bicolor; Jiang et al., 2013), and wheat (Triticum aestivum; Wang et al., 2014).”
• Genome Editing of Plants (2017, background): “Another example of mutagenesis in complex genomes was described in hexaploid wheat, in which six alleles of the Mlo gene were knocked out, thereby conferring resistance to the fungus that causes powdery mildew (Wang et al., 2014).”
• Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN] (2015, background): “Similar observations have been previously reported in several plant species (Brooks et al., 2014; Feng et al., 2014; Jiang et al., 2014; Wang et al., 2014; Zhang et al., 2014).”
• Genetically engineered crops help support conservation biological control (2019, methodology): “The technique has already been successfully applied to crop plants to alter agriculturally important traits such as disease resistance (Wang et al., 2014) and drought tolerance (Shi et al.”
• The CRISPR/Cas9 system for plant genome editing and beyond. (2015, methodology): “Subsequent work focused on additional crop species such as sorghum (Jiang et al., 2013b), wheat (Upadhyay et al., 2013; Wang et al., 2014b) andmaize (Liang et al., 2014).”
• A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, methodology): “To promote TALEN technology, we developed a streamlined TALEN assembly method (Cermak et al., 2011), which was later used for successful genome editing in many plant species (Christian et al., 2013; Shan et al., 2013a;Wendt et al., 2013; Zhang et al., 2013; Lor et al., 2014; Wang et al., 2014).”
• Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA (2016, positive): “Under our selection-free conditions, the total time from particle bombardment to obtaining testable plants was 6–8 weeks, which is 2–4 weeks shorter than the genome-editing protocol reported previously 9 .”