Subsets of Myeloid-Derived Suppressor Cells in Tumor Bearing Mice

By Je-in Youn, S. Nagaraj, Michelle Collazo, D. Gabrilovich. Journal of Immunology, 2008

1,715 citations recorded by the source corpus.

Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of cells that play a critical role in tumor associated immune suppression. In an attempt to identify a specific subset of MDSC primarily responsible for immunosuppressive features of these cells, 10 different tumor models were investigated. All models showed variable but significant increase in the population of MDSC. Variability of MDSC expansion in vivo matched closely the effect of tumor cell condition medium in vitro. MDSC consists of two major subsets of Ly6G+Ly6Clow granulocytic and Ly6G−Ly6Chigh monocytic cells. Granulocytic MDSC have increased level of reactive oxygen species and undetectable level of NO whereas monocytic MDSC had increased level of NO but undetectable levels of reactive oxygen species. However, their suppressive activity per cell basis was comparable. Almost all tumor models demonstrated a preferential expansion of granulocytic subset of MDSC. We performed a phenotypical and functional analysis of several surface molecules previously suggested to be involved in MDSC-mediated suppression of T cells: CD115, CD124, CD80, PD-L1, and PD-L2. Although substantial proportion of MDSC expressed those molecules no differences in the level of their expression or the proportion, positive cells were found between MDSC and cells from tumor-free mice that lack immune suppressive activity. The level of MDSC-mediated T cell suppression did not depend on the expression of these molecules. These data indicate that suppressive features of MDSC is caused not by expansion of a specific subset but more likely represent a functional state of these cells.

Subfields: Immunology, Neurology

View Paper: https://www.semanticscholar.org/paper/edcce60578b86776d1a00480857ca203f0a55120

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Immunology

1. D. Gabrilovich
2. V. Bronte
3. T. Condamine
4. Paola Zanovello
5. L. Dolcetti
6. C. Rosales
7. Je-in Youn
8. Ilaria Marigo

In Neurology

1. D. Gabrilovich
2. V. Bronte
3. Paola Zanovello
4. J. Massagué
5. E. Batlle

Citations in the corpus

• HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment (2010, background): “Two major groups of MDSCs are now identified: cells with granulocytic (CD11b+Ly6G+Ly6Clow) and monocytic (CD11b+Ly6GLy6Chigh) phenotype (Movahedi et al., 2008; Youn et al., 2008).”
• Tumor entrained neutrophils inhibit seeding in the premetastatic lung (2011, neutral): “These features are common the both TENs and granulocytic myeloid derived suppressor cells (G-MDSCs) (Kusmartsev and Gabrilovich, 2006; Youn et al., 2008) and raise the interesting possibility that TENs might also have immune suppressive qualities or alternatively represent a distinct subpopulation…”
• Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients (2016, background): “Two large populations of MDSCs are currently described: polymorphonuclear MDSCs (PMN-MDSCs) and monocytic MDSCs (MMDSCs) (13).”
• Phenotypic diversity and plasticity in circulating neutrophil subpopulations in cancer. (2015, background): “…switching to become LDNs in a TGFb-dependent fashion, a switch accompanied by loss of antitumor properties and the gain of immunosuppressive properties, similar to what has been described for immature granulocytic myeloid-derived suppressor cells (G-MDSCs) (Waight et al., 2011; Youn et al., 2008).”
• Tumor-associated neutrophils: friend or foe? (2012, neutral): “large quantities in the spleens of tumor-bearing animals [10-13] and for tumor-”
• Myeloid-derived suppressor cell heterogeneity and subset definition. (2010, methodology): “References [23,24,25 ] provide a thorough analysis of mouse MDSC subsets in different models of tranplantable tumors.”
• Tumor-induced tolerance and immune suppression depend on the C/EBPbeta transcription factor. (2010, background): “We identified the two major MDSC subsets: CD11b+Gr-1hiCD62L+F4/80+/ granulocytic and CD11b+Gr-1lo CD62L F4/80+ monocytic cells, as previously described (Gabrilovich and Nagaraj, 2009; Sica and Bronte, 2007; Youn et al., 2008).”