Diet-Dependent Shifts in the Bacterial Population of the Rumen Revealed with Real-Time PCR

By K. Tajima, R. Aminov, T. Nagamine, H. Matsui, M. Nakamura, Y. Benno. Applied and Environmental Microbiology, 2001

692 citations recorded by the source corpus.

Subfields: Agronomy and Crop Science, Ecology, Genetics

View Paper: https://www.semanticscholar.org/paper/e5e717f0a954236a20df5ac6ef19349ad8a5292f

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Citations in the corpus

Rumen microbial ecosystem (2005, methodology): “Tajima et al.(97) designed and revalidated PCR primers for the detection of 13 species of rumen bacteria and these primers have been used with real-time PCR for quantification of these bacteria in the rumen microbial eco-system.”
Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR (2007, positive): “Our data also find analogy with the real-time PCR study of Tajima et al. (2001) who reported that 16S rRNA gene copies of Prevotella species far exceeded those of 11 other bacterial species (including 8 species examined in our study).”
Control of Rumen Microbial Fermentation for Mitigating Methane Emissions from the Rumen (2008, background): “It has been reported that the numbers of lactate-producing and lactate-utilizing bacteria in the rumen change during adaptation from a low- to a high-concentrate diet (Mackie and Gilchrist, 1979; Tajima et al., 2001).”
Ruminal acidosis in beef cattle: the current microbiological and nutritional outlook. (2007, methodology): “Tajima et al. (2001) used real-time quantitative PCR for specific detection and quantification of 13 species of ruminal bacteria in the rumens of cows switched from a hay to a grain diet.”
Structure of the Archaeal Community of the Rumen (2008, background): “Diet is known to affect the composition of the bacterial community in the rumen (19, 47), but bacteria can be expected to interact more intimately with the diet since they will be using mainly feed components as their energy substrates.”
Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. (2006, methodology): “The F. succinogenes primer set described here produces a smaller amplicon (121 bp) than that previously described (445 bp) (Tajima et al., 2001).”
Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice (2010, background): “It is not surprising that significant and long-term changes in diet composition will lead to profound alterations in gut microbiota structure (Finegold et al., 1974; Tajima et al., 2001).”
Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces (2004, methodology): “Although conventional PCR does not permit quantitative detection of target bacteria, real-time PCR with species-specific primers can provide precise quantification through measurement of SYBR Green I fluorescence to determine the amounts of PCR products in each cycle (11, 14, 16, 17, 24, 29).”
Development of an extensive set of 16S rDNA‐targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real‐time PCR (2004, background): “…applied for quantification of bacterial DNA in various environments such as faeces (Requena et al. 2002; Bélanger et al. 2003; Malinen et al. 2003), colonic tissue (Fujita et al. 2002), rumen (Tajima et al. 2001), gastric tissue (He et al. 2002) and periodontal samples (Asai et al. 2002).”
Low Ruminal pH Reduces Dietary Fiber Digestion via Reduced Microbial Attachment (2006, background): “Sci. 20(2):200-207 201 apparent that low ruminal pH changes the rumen microbial population from fibrolytic to amylolytic (Tajima et al., 2001).”
Rumen Microbial Population Dynamics during Adaptation to a High-Grain Diet (2010, background): “Fibrobacter succinogenes is a fibrolytic bacterium that digests fiber (24, 46) and is predominantly present in diets high in fiber.”