The ground state of embryonic stem cell self-renewal

By Qi-Long Ying, Jason Wray, Jennifer Nichols, L. Batlle-Morera, B. Doble, J. Woodgett, P. Cohen, Austin G Smith. Nature, 2008

3,530 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e44aa80c6e4be7b483789525b572596ccce0ad87

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Citations in the corpus

Induced pluripotency: history, mechanisms, and applications. (2010, background): “Of note, the combined inhibition of Gsk3 and MAPK signaling has been shown previously by the Smith laboratory (Ying et al. 2008) to be sufficient to maintain murine ESCs in a self-renewing, pluripotent state by suppressing their differentiation.”
Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo (2009, methodology): “The combination of PD0325901 with Chir99021, termed 2i, is very effective for propagating ES and induced pluripotent cells (Buehr et al., 2008; Silva, J. et al., 2008; Ying et al., 2008).”
SOX17 is a Critical Specifier of Human Primordial Germ Cell Fate (2014, methodology): “…and filtered with 50 mm cell filter (PERTEC), and 43 105 cells/ 12-well were plated on vitronectin/gelatin-coated plates (Gafni et al., 2013) in N2B27 medium (Ying et al., 2008) with 1% KSR, 10 ng/ml bFGF (SCI), 1 ng/ml TGF-b1 (Peprotech), or 20 ng/ml Activin A (SCI) and 10 mM ROCK inhibitor.”
Reversing DNA Methylation: Mechanisms, Genomics, and Biological Functions (2014, positive): “GSK3, known as 2i condition), is sufficient to maintain mouse ESCs in the naive ICM-like state (Ying et al., 2008).”
Wnt signaling and stem cell control (2008, background): “of undifferentiated ES cells, bypassing the requirement for LIF/STAT3 (Ying et al. 2008).”
Proliferation, survival and metabolism: the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination (2016, background): “Active MAPK/ERK signalling is a key requirement for mESCs to undergo differentiation (Ying et al., 2008); thus, inhibition of MAPK/ERK signalling by LIF-induced PI3K may contribute to the maintenance of pluripotency even though the exact mechanisms by which PI3K inhibits MAPK/ERK are unclear…”
Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts (2008, positive): “3i can support efficient derivation and maintenance of ES cells from different strains of mice (Ying et al., 2008).”
Experience dictates stem cell fate in the adult hippocampus. (2011, neutral): “…with this notion, indelible lineage analyses from several adult nonneuronal stem cell systems have suggested that changes in the stem cell niche result in modified lineage trajectories and tissue homeostasis (Clayton et al., 2007; Jones et al., 2007; Nakagawa et al., 2007; Ying et al., 2008).”
Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition (2008, methodology): “Our study has also revealed that the initial products in the reprogramming process, partially reprogrammed cells [1,2], can be promoted to full pluripotent status by applying simple, molecularly defined conditions established for derivation and propagation of ES cells [11].”
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. (2009, positive): “The resulting hiPSCs appear to be homogenous and self-renew over long-term periods (>20 passages) in the presence of hLIF and the chemical cocktail of PD0325901, A-83-01, and CHIR99021 (Figure 2B).”
Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency (2014, neutral): “First, while the culture conditions described here generate pluripotent cells that more closely approximate the naive state of mouse ESCs, they fall short of establishing a ground state in which self-renewal is fully independent of extrinsic instruction (Ying et al., 2008).”
A double take on bivalent promoters. (2013, background): “Interestingly, the requirement for serum can be bypassed through the use of two small-molecule inhibitors (2i conditions) (Ying et al. 2008; Wray et al. 2011).”