Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts
By Ping Li, C. Tong, R. Mehrian-Shai, L. Jia, Nancy L. Wu, Youzhen Yan, R. Maxson, E. Schulze, Hou-yan Song, C. Hsieh and 2 more. Cell, 2008
703 citations recorded by the source corpus.
SUMMARY Rats have important advantages over mice as an experimental system for physiological and pharmacological investigations. The lack of rat embryonic stem (ES) cells has restricted the availability of transgenic technologies to create genetic models in this species. Here, we show that rat ES cells can be efficiently derived, propagated, and genetically manipulated in the presence of small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. These rat ES cells express pluripotency markers and retain the capacity to differentiate into derivatives of all three germ layers. Most importantly, they can produce high rates of chimerism when reintroduced into early stage embryos and can transmit through the germline. Establishment of authentic rat ES cells will make possible sophisticated genetic manipulation to create models for the study of human diseases.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/d564775c8b14b15e9f2b0e8a32f0ecc646807ed8
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In Molecular Biology
- Jennifer Nichols
- José C. R. Silva
- Austin G Smith
- M. Roode
- H. Stunnenberg
- G. Keller
- Richard A. Young
- S. Yamanaka
Citations in the corpus
- The Transcriptional and Epigenomic Foundations of Ground State Pluripotency (2012, background): “Use of 2i has enabled derivation of germline-competent ES cells from all mouse strains tested and for the first time from rats (Buehr et al., 2008; Hanna et al., 2009; Kiyonari et al., 2010; Li et al., 2008; Nichols et al., 2009).”
- Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo (2009, methodology): “Authentic ES cells are characterised by the unusual capacity to proliferate in the presence of selective inhibition of the Mek/Erk1/2 signalling pathway (Buehr et al., 2008; Burdon et al., 1999; Chen et al., 2006; Kunath et al., 2007; Li et al., 2008; Ying et al., 2008).”
- Naive and primed pluripotent states. (2009, methodology): “Rat ESCs capable of chimera contribution and germline transmission have finally been derived (Buehr et al., 2008; Li et al., 2008).”
- Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency (2014, neutral): “This suggests that naive human ESCs may be susceptible to acquiring chromosomal abnormalities, similar to previous observations with naive rat ESCs (Li et al., 2008).”