Chimeric Nucleases Stimulate Gene Targeting in Human Cells

By M. Porteus, David Baltimore. Science, 2003

766 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e1ca3890df23d30518aaabaa0963a2c58ac313f3

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In Molecular Biology

1. D. Carroll
2. Feng Zhang
3. J. Keith Joung
4. Daniel F. Voytas
5. Jin-Soo Kim
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7. A. Klug
8. Nathan D. Lawson

Citations in the corpus

• Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, background): “2013), rapidly catching up with their precursors, namely, zinc finger nucleases (ZFNs) (Bibikova et al. 2003; Porteus and Baltimore 2003) and transcription activator-like effector nucleases (TALENs) (Miller et al.”
• The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution. (2004, background): “The application of zinc finger nucleases for gene targeting in vertebrates has already been demonstrated (Bibikova et al., 2003; Porteus and Baltimore, 2003) and it may be only a matter of time before the first reports of the use of the technique will appear for plants.”
• Gene targeting using zinc finger nucleases (2005, methodology): “Under optimized conditions, targeting rates of 3–5% have been achieved using a reporter gen”
• Targeting DNA Double-Strand Breaks with TAL Effector Nucleases (2010, methodology): “As a positive control we used a wellcharacterized zinc finger nuclease with a DNA binding domain derived from the mouse transcription factor Zif268 (Porteus and Baltimore 2003).”
• Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases (2008, methodology): “In vertebrate cell lines, genomic lesions at a specific site have been introduced using zinc-finger nucleases (ZFNs”
• TALENs: a widely applicable technology for targeted genome editing (2012, neutral): “…38 , catfish 39 , sea urchin 40 , frog 41 , pig 42 , cattle 43 , cricket 8 , rabbit 44 , silkworm 45 , butterfly 46 , mouse 47, 48 , rat 49, 50 , soybean 51, 52 , thale cress 53, 54 , corn 55 , tobacco 56 , petunia 57 , hamster cells 58 , and human somatic 59, 60 and pluripotent stem cells 61–63 .”
• Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification (2008, neutral): “In addition, the variant VF2468 ZFN pair revealed minimal toxicity (Figure 4D) similar to that of I-SceI, a highly specific meganuclease used previously as a control (Porteus and Baltimore, 2003).”
• Genome Engineering of Crops with Designer Nucleases (2012, background): “Two landmark papers demonstrated the utility of ZFNs for making targeted modii cations to the Drosophila melanogaster and human (Homo sapiens sapiens) genomes (Bibikova et al., 2003; Porteus and Baltimore, 2003).”
• CRISPR/Cas9 Beta-globin Gene Targeting in Human Hematopoietic Stem Cells (2016, neutral): “c, Colonies were scored according to their morphology: (1) CFUerythroid (CFU-E); (2) burst forming unit-erythroid (BFU-E); (3) CFU-granulocyte/macrophage (CFU-GM); and (4) CFU-granulocyte/ erythrocyte/macrophage/megakaryocyte (CFU-GEMM) (n = 2 different HSPC donors).”