TALENs: a widely applicable technology for targeted genome editing

By J. Keith Joung, Jeffry D. Sander. Nature reviews. Molecular cell biology, 2012

1,546 citations recorded by the source corpus.

Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/bdbb6ebf14955bf86343179218c465331ca38516

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Citations in the corpus

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System1 (2014, positive): “Two of these strategies, zinc finger nucleases (Urnov et al., 2010) and transcription activator-like nucleases (TALENs; Joung and Sander, 2013), are based on protein-DNA interactions, whereas a third technology, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated9…”
Biology and Diseases of Mice (2015, neutral): “TALENs function similarly, but are composed of tandem repeats of 33-35 amino acids, each with nucleotide specificity occurring in two hypervariable amino acids, the 'repeat variable di-residue (RVD)', at positions 12 and 13 (Joung and Sander, 2013) .”
Delivering CRISPR: a review of the challenges and approaches (2018, background): “For an in-depth TALEN function review, see Joung & Sander (2013).”
CRISPR-Cas9 mediated mutagenesis of a DMR6 ortholog in tomato confers broad-spectrum disease resistance (2016, methodology): “NHEJ repair can cause insertions or deletions (indels) around the DNA breaks, whereas HR can repair double-strand breaks using a homologous repair template (Joung and Sander, 2013; Liu and Fan, 2014; Urnov et al. , 2010).”
Loss-of-function approaches in comparative physiology: is there a future for knockdown experiments in the era of genome editing? (2019, background): “Examples of LOF studies in comparative physiology Model system LOF approach Delivery method LOF induced Experiment performed References Invertebrates Nematode (Caenorhabditis elegans) RNAi E. coli feeding Embryo/ larva Embryo to larva Choe and Strange, 2008; Crombie et al., 2016 CRISPR/Cas9 Plasmid ”
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system (2013, background): “R methodological innovations that exploit zinc finger nucleases (ZFNs) and transcription-activator–like effector nucleases (TALENs) have made it possible to introduce sitespecific genomic modifications in cell culture systems and in a wide range of species (1, 2).”
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis (2013, neutral): “Biochem Biophys Res Commun 349(4):1269–1277.”