Gene targeting using zinc finger nucleases

By M. Porteus, D. Carroll. Nature Biotechnology, 2005

641 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/ff44d1275c12f59b96bf4e2b1e33c2df212e8dba

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In Molecular Biology

Citations in the corpus

New plant breeding techniques. State-of-the-art and prospects for commercial development (2011, background): “Furthermore, sustained expression of ZFNs is likely to contribute to cellular toxicity due to non-specific binding leading to unwanted DSBs in the genome (Porteus and Carroll, 2005).”
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery (2007, methodology): “Recently, ZFNs have been used to enhance the frequency of gene correctio”
Efficient TALEN-mediated gene knockout in livestock (2012, background): “Lesions are often repaired by nonhomologous end-joining (NHEJ) that typically results in small insertions or deletions (indels) (5), two-thirds of which cause a frame-shift that disables encoded proteins.”
Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification (2008, positive): “ZFNs function as dimers with each monomer composed of a non-specific cleavage domain from the FokI endonuclease fused to a zinc-finger array engineered to bind a target DNA sequence of interest (Cathomen and Joung, 2008; Durai et al., 2005; Porteus and Carroll, 2005).”
TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain (2010, methodology): “To date the most promising method involving genome editing is the use of custom-designed zinc finger nucleases (ZFNs) (5).”
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases (2008, methodology): “In vertebrate cell lines, genomic lesions at a specific site have been introduced using zinc-finger nucleases (ZFNs”
Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease (2007, background): “This is a particularly attractive feature of homing endonucleases, in contrast to other potential genome engineering reagents such as zinc finger nucleases (11).”
Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. (2013, background): “…to yield indels (Barnes, 2001) or by homologous recombination (HR) if homologous donor templates are available (van den Bosch et al., 2002), thereby enhancing the efficiency of HR-based gene targeting (Bibikova et al., 2003; Porteus and Carroll, 2005; Thomas and Capecchi, 1987; Urnov et al., 2005).”