Genome Engineering of Crops with Designer Nucleases
By Shaun J. Curtin, Daniel F. Voytas, R. Stupar. 2012
117 citations recorded by the source corpus.
Recent advances in the field of genome engineering indicate that it will soon be routine to make site‐directed modifications to the genomes of crop species, including targeted mutations, gene insertions, and gene replacements. This new technology will be used to help elucidate gene function and develop new and valuable traits. Key to enabling site‐directed genome modifications are sequence‐specific nucleases that generate targeted double‐stranded DNA breaks in genes of interest. To date, three different sequence‐specific nuclease systems have been used in crop plants: zinc finger nucleases, transcription activator‐like effector nucleases (TALENs), and LAGLIDADG homing endonucleases, also termed “meganucleases.” In this review, we report on the current state of genome engineering in crop plants, comparing the different nuclease and gene delivery systems. We also consider some of the limitations that nuclease‐mediated crop improvement technologies may encounter.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/c1ea5cb4c468fc8c624d7052b690e7b9c58b076a
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
In Plant Science
Citations in the corpus
- Salt resistant crop plants. (2014, background): “Designer nucleasebased approaches to alter genetic composition, such as Transcription Activator-Like Effector Nucleases (TALEN) [82–85], are reported to increase the mutagenesis efficiency of endogenous targets by specifying their binding sites with single nucleotide precision [85].”