Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
By Xiangdong Meng, Marcus B. Noyes, Lihua Julie Zhu, Nathan D. Lawson, Scot A. Wolfe. Nature Biotechnology, 2008
804 citations recorded by the source corpus.
Subfields: Cell Biology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/f14872258f7e018e7455b9a6478a7b1ed65531bb
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Citations in the corpus
- Genome Engineering With Zinc-Finger Nucleases (2011, methodology): “This is a wellestablished method in zebrafish, and very usable frequencies of ZFN-induced mutagenesis have been achieved in quite a number of genes (Doyon et al. 2008; Meng et al. 2008; Foley et al. 2009).”
- TALENs: a widely applicable technology for targeted genome editing (2012, neutral): “Additional methods that explicitly account for context-dependent effects among zinc fingers in an array include: Oligomerized Pool Engineering ( OPEN ) 90 , Context-Dependent Assembly ( CoDA ) 52 , and a bacterial one-hybrid ( B1H ) selection-based system 37 .”
- Reverse genetic screening reveals poor correlation between Morpholino-induced and mutant phenotypes in zebrafish (2014, methodology): “Initial work utilized zinc finger nucleases (ZFNs; Doyon et al., 2008; Meng et al., 2008), and transcription activator-like effector nucleases (TALENs), which provide improved specificity over ZFNs, also have been developed (Cade et al., 2012).”
- Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells (2011, methodology): “The p62 in both cell lines was successfully deleted using a zinc finger nuclease system comprised of a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at specified locations (Meng et al., 2008; Santiago et al., 2008).”
- Genome editing with engineered zinc finger nucleases (2010, background): “In four separate studies up to 50% germline mosaicism at the targeted genes was obtaine”
- A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity (2011, background): “Accordingly, designer nuclease-based genome engineering has been successfully established in more than 10 model organisms thus far, including plants (3,4), invertebrates (5), fish (6,7) and mammals (8).”
- Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs (2014, methodology): “We adapted a previously implemented algorithm to determine indel frequency [19], and improved it to account for technical artifacts and sequencing errors (see Materials and Methods).”