Establishment and Maintenance of Genomic Methylation Patterns in Mouse Embryonic Stem Cells by Dnmt3a and Dnmt3b

By Taiping Chen, Y. Ueda, J. E. Dodge, Zhenjuan Wang, E. Li. Molecular and Cellular Biology, 2003

754 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Pediatrics, Perinatology and Child Health

View Paper: https://www.semanticscholar.org/paper/e038def90f8da1359890832f422e28acd34fe877

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In Genetics

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Citations in the corpus

• Dnmt1 and Dnmt3a are required for the maintenance of DNA methylation and synaptic function in adult forebrain neurons (2010, background): “In mouse embryonic stem cells, Dnmt1 cooperates with Dnmt3a and Dnmt3b to maintain DNA methylatio”
• Rethinking how DNA Methylation Patterns are Maintained (2009, background): “For example, mouse embryonic stem (ES) cells that contain the maintenance enzyme DNMT1 as the sole DNMT show virtually no methylation of imprinted genes and repeats, and they gradually lose the methylation of other sequences with increasing numbers of division”
• Reversing DNA Methylation: Mechanisms, Genomics, and Biological Functions (2014, positive): “In support of this notion, mouse ESCs deficient in both DNMT3A and DNMT3B become globally hypomethylated after prolonged culturing (Chen et al., 2003).”
• Dnmt3a is essential for hematopoietic stem cell differentiation (2011, background): “Embryonic stem (ES) cells lacking both Dnmt3a and Dnmt3b progressively lose differentiation potential with cell passag”
• Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis (2005, background): “Although early passage Dnmt3a/b double mutant ES cells show almost wild-type levels of CpG methylation (43,44), they progressively lose methylation with <1% remaining after 75 passages (44).”
• How is pluripotency determined and maintained? (2007, background): “5 NT Normal Normal Defective Restored Okano et al., 1999; Chen et al., 2003 Dnmt1/3a/3b NT NT Normal Normal Defective Restored Tsumura et al., 2006 Dnmt3l Viable Normal Normal Normal Normal NT Hata et al., 2002 Cgbp (Cxxc1) Die at E6.”
• miR-148 targets human DNMT3b protein coding region. (2008, background): “Murine DNMT3b3 is catalytically inactive both in vitro (Aoki et al. 2001) and in vivo (Chen et al. 2003); however, the DNA methyltransferase activity of human DNMT3b3 depended on which substrate was chosen (Soejima et al. 2003; Chen et al. 2005).”
• Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. (2005, background): “…evidence that they also require an intact RNAi pathway: (1) In DCR ⌬/⌬ cells, DNA methylation was partially abolished at the minor satellite repeats, similar to dnmt3a −/− 3b −/− ES cells (Chen et al. 2003) and (2) histone di-and trimethylation were reduced in a large percentage of the cells.”
• DNA methyltransferases control telomere length and telomere recombination in mammalian cells (2006, background): “Three active DNMTs (DNMT1, DNMT3a and DNMT3b) have been identified in human and mous”
• Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states. (2008, background): “Furthermore, this is consistent with the evidence that de novo DNA methylation is not essential for self-renewal of ESCs but is essential for their differentiation ( Chen et al., 2003; Lei et al., 1996).”