Identification of essential genes in cultured mammalian cells using small interfering RNAs.

By J. Harborth, Sayda M. Elbashir, Kim Bechert, Thomas Tuschl, K. Weber. Journal of Cell Science, 2001

887 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/dcb4a4871d78a1497aa8010f47faa0f7f669a81c

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Citations in the corpus

Gene silencing in mammals by small interfering RNAs (2002, background): “Although this question has not been systematically studied, moderately to highly expressed genes, such as the nuclear lamin genes, can be knocked down to undetectable level”
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. (2006, unknown): “Furthermore, cells expressing shTEK-1275 and shTEK-520 also showed altered DNA content distribution, consistent with G2/M arrest (Figure 6L).”
The ARP2/3 complex: an actin nucleator comes of age (2006, background): “Although there are no published reports of ARP2/3-knockout mammals, the inactivation of ARPC3 in human HeLa cells by RNAi is letha”
How complete are current yeast and human protein-interaction networks? (2006, background): “…protein (the ‘degree’) is plotted for 907 essential vertebrate proteins known from mouse knockouts [50], human small interfering (si)RNA screens [51,52], and zebrafish random mutagenesis [53] and for the remaining 6,661 proteins in the network, considering only the largest connected network…”
Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin. (2008, unknown): “These studies have shown that down-regulation of either lamin B1 or B2 induces apoptosis in HeLa cells (Harborth et al. 2001).”
Lamin B1 loss is a senescence-associated biomarker (2012, methodology): “Mice were treated and mRNA was collected and analyzed as described in Figure 4F.”
Identification of the gene for vitamin K epoxide reductase (2004, methodology): “siRNA transfection Transfection was essentially as described previousl”
Functional and physical interaction between Bcl‐XL and a BH3‐like domain in Beclin‐1 (2007, background): “…Vps (siRNA1 from Nobukuni et al, 2005 and siRNA2 from Byfield et al, 2005), a scrambled siRNA or an siRNA targeting the unrelated protein emerin (Harborth et al, 2001). siRNA effects were controlled by immunoblots with suitable antibodies specific for Beclin-1 (SantaCruz), Bad (SantaCruz) or…”