Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture

By Qi-Long Ying, Marios P. Stavridis, Dean S. Griffiths, M. Li, Austin G Smith. Nature Biotechnology, 2003

1,591 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d600f298ef66a53f918e0a64ef0615be18f84255

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Citations in the corpus

CDK8/9 drive Smad transcriptional action, turnover and YAP interactions in BMP and TGFβ pathways (2009, positive): “assays were carried out as described ( Ying et al., 2003b ) in the presence or absence of BMP2 (25 ng/ml, R&D Systems).”
Capture of authentic embryonic stem cells from rat blastocysts. (2008, positive): “for mouse ES cells (Ying et al., 2003b) except that laminin was used as”
Reciprocal Transcriptional Regulation of Pou5f1 and Sox2 via the Oct4/Sox2 Complex in Embryonic Stem Cells (2005, background): “In addition, ESCs can also differentiate into a variety of different cell types when cultured in vitro (17, 19, 20, 40, 52).”
Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses (2012, methodology): “Results Differentiation to cortical stem cells is retinoid dependent We explored a number of different approaches to direct differentiation of human ES cells to neural stem and progenitor cells of the cerebral cortex in monolayer culture, starting from previously described methods for neura”
Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells (2011, background): “…media allows BMP to switch from supporting self-renewal to inducing mesodermal and endodermal differentiation while blocking entry into neural lineages; when grown in the absence of both LIF signals and serum, ESCs are predisposed to convert to a neuronal fate (Ying et al., 2003a, 2003b).”
Specification of motoneurons from human embryonic stem cells (2005, methodology): “, have been induced in vitro to adopt a neuroectodermal fat”
The Transcriptional and Epigenomic Foundations of Ground State Pluripotency (2012, methodology): “They may confer additional robustness to the pluripotent state to counter differentiation stimuli (Ying et al., 2003a).”
Oct4-induced pluripotency in adult neural stem cells. (2009, positive): “We derived NSCs from 1F iPS in serum-free adherent monolayer cultures according to a published protocol (Ying et al., 2003).”
An extracellular matrix microarray for probing cellular differentiation (2005, background): “Exceptions to this generalization are a few rare reports of efficient monolayer culture methods, underscoring the importance of a tightly regulated environment for efficient lineage-specific differentiatio”
Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell (2005, methodology): “We induced neural precursor differentiation from ES cells in serum-free adherent monoculture [35,36].”
Opposing FGF and retinoid pathways control ventral neural pattern, neuronal differentiation, and segmentation during body axis extension. (2003, background): “A striking analogy can be drawn with the proximoWichterle et al., 2002; Ying et al., 2003); however, it is distal extension of the limb in which distal FGF signaling not known whether these pathways interact to regulate provided by the apical ridge restricts RA synthesis and these activities.”
Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. (2008, background): “Neuroectoderm induction is inhibited by BMP, Wnt, and activin/Nodal signaling, consistent with the observation that these pathways are not active in the region of ectoderm induction in the early embryo (Aubert et al., 2002; Kubo et al., 2004; Ying et al., 2003).”