Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells.

By D. Kurz, S. Decary, Ying Hong, J. Erusalimsky. Journal of Cell Science, 2000

813 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/d233471aa9939c96a6522338dcad0c2b147910d5

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Citations in the corpus

• The essence of senescence. (2010, background): “Furthermore, one would expect that increases in the number or activity of lysosomes unrelated to senescence would also result in elevated lysosomal b-galactosidase protein levels, and thus in increased SA-b-GAL activity.”
• Foxo3 circular RNA promotes cardiac senescence by modulating multiple factors associated with stress and senescence responses (2016, background): “This is characterized by a flattened and enlarged cell morphology, and lysosomal activation, resulting in positive staining of senescence-associated b-galactosidase activity (SA-b-gal).(6) Stress can induce premature senescence and activate many markers similar to those in replicative senescence.”
• Programmed cell senescence during mammalian embryonic development. (2013, unknown): “SAbG is the most widely used assay to detect senescence both in vitro and in vivo (Collado and Serrano, 2006; Dimri et al., 1995), and it is based on the increased lysosomal content of senescent cells (Kurz et al., 2000).”
• DNA damage response and cellular senescence in tissues of aging mice (2009, result): “…activity at pH6 (senescence-associated b-galactosidase or Sen-b-gal; Dimri et al., 1995), which was not causally related to senescence induction (Lee et al., 2006), was cumbersome at least in some tissues and resulted not too seldom in contradictory data (Kurz et al., 2000; Severino et al., 2000).”
• Senescence‐associated β‐galactosidase is lysosomal β‐galactosidase (2006, background): “Our results, together with published results from other laboratories (Kurz et al., 2000; Gerland et al., 2003; Gary & Kindell, 2005; Yang & Hu, 2005), demonstrate that lysosomal β-galactosidase activity increases enough in senescent cells to be detected at suboptimal pH 6.0, constituting SA-β-Gal…”
• Aging of mesenchymal stem cells. (2006, background): “One theory suggests that beta-GAL activity is associated with the RAS pathway (Minamino et al., 2003) and with lysosomal dysfunction (Kurz et al., 2000).”
• ISMARA: automated modeling of genomic signals as a democracy of regulatory motifs (2014, background): “Lysosomes are responsible for the degradation of many macromolecules, including proteins, and increase in lysosomal mass is a well-known characteristic of aging and senescence in cells (Cuervo and Dice 2000; Kurz et al. 2000).”
• Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “5) becomes detectable as result of increased lysosomal content (Kurz et al., 2000).”