Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment.

By R. McBeath, Dana M. Pirone, C. Nelson, Kiran Bhadriraju, Christopher S. Chen. Developmental Cell, 2004

4,106 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b2d9ef927b8ea635d7f624b50c6e37ece4b110d7

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Citations in the corpus

Mechanical control of tissue and organ development (2010, background): “…that are coated with ECM proteins decide their fate depending on the elasticity of their ECM substrate, even when cultured in the presence of soluble inducing factors, and this mechanical control mechanism depends on the generation of Rho-dependent cytoskeletal tension (McBeath et al., 2004).”
Mechanical control of stem cell differentiation (2008, background): “Nonetheless, it is also important to acknowledge that in some cases mechanical signals appear to completely bypass the influence of soluble factors by directly altering gene expression (Farge, 2003; McBeath et al., 2004; Jakkaraju et al., 2005; Engler et al., 2006).”
Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy (2004, background): “, Cbfa1/Runx2, PPARγ, Sox9, and MEF2) play important roles in driving the commitment of multipotent stem cells into specific lineages, and maintain their differentiated phenotypes [63-66].”
The biology of YAP/TAZ: hippo signaling and beyond. (2014, background): “Mesenchymal stem cells (MSC) can differentiate into distinct cell types depending on the stiffness of the ECM in which they are cultured (54, 137).”
Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC (2011, background): “For preadipocyte differentiation of AT-MSC (ASC) a high cellular density and a subsequent growth arrest at the G0/G1 boundary are important [64,65].”
Stem cell fate dictated solely by altered nanotube dimension (2009, background): “(28) reported that initial cell-plating density made critical effects on the differentiation of mesenchymal stem cells, and a lower cell density showed better osteoblastic differentiation compared with a higher cell density in the presence of osteogenic inducing media.”
An algorithm-based topographical biomaterials library to instruct cell fate (2011, background): “Using micro pattering techniques, Chen and coworkers showed that the decision of a mesenchymal stromal cell (MSC) to either become a fat or a bone cell depends on the shape the cell, which correlated with the activation of the RhoA signaling pathway (6).”
Surface engineering approaches to micropattern surfaces for cell-based assays. (2006, background): “[9] McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS.”
Integrin-mediated mechanotransduction (2016, background): “…cytochalasin D or the ROCK inhibitor Y-27632 or by expression of a dominant-negative RhoA promotes adipogenesis on rigid, osteogenic conditions, whereas overexpression of constitutively active ROCK restores osteogenic fate on soft, adipogenic conditions (McBeath et al., 2004; Engler et al., 2006).”
Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion (2005, background): “Combined with recent evidence documenting RhoA’s central role in the shape-induced regulation of mesenchymal stem cell commitment (McBeath et al., 2004), these findings support the argument that the regulation of RhoA activity by mechanical forces may be critical to predictably influence cell…”