Fragile X-related protein and VIG associate with the RNA interference machinery.

By Amy A. Caudy, Mike Myers, Gregory J. Hannon, Scott M. Hammond. Genes & Development, 2002

671 citations recorded by the source corpus.

RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Drosophila homolog of the fragile X mental retardation protein (FMRP), dFXR, and VIG (Vasa intronic gene), through their association with RISC. FMRP, the product of the human fragile X locus, regulates the expression of numerous mRNAs via an unknown mechanism. The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease.

Subfields: Cancer Research, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b0c3d90603302bc6d1fd3a63d386f02a445722f9

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Citations in the corpus

Human MicroRNA Targets (2004, background): “Independently, FMRP has been shown to be associated with RISC components and miRNAs (Jin et al. 2004).”
A microRNA array reveals extensive regulation of microRNAs during brain development. (2003, background): “Mutation of this protein causes mouse neuromuscular degeneration (Cox et al. 1998), a disease similar to human spinal muscular atrophy and linked to miRNA-containing complex (see above).”
Processing of primary microRNAs by the Microprocessor complex (2004, methodology): “Methods Drosophila cell culture and extract preparation Drosophila S2 cells were cultured as described previousl”
Mechanisms of gene silencing by double-stranded RNA (2004, background): “melanogaster RISC: the Vasa intronic gene product (VIG), fragile-X-related protein (dFXR), and the tudor staphylococcal-nuclease-domain-containing protein (Tudor-SN”
The mGluR theory of fragile X mental retardation. (2004, positive): “Specifically, FMRP is partof a RISC nucleasecomplex that represses translation by directing small interfering RNAs (siRNA) to their mRNA targets [ 47 ,48].”
Specificity of microRNA target selection in translational repression. (2004, background): “Additional factors may also be important for determining in vivo targets of miRNAs, such as the FMRP protein, a known regulator of mRNA translation that has been implicated in RNA silencing complexes (Caudy et al. 2002; Ishizuka et al. 2002).”
RNA Interference: Biology, Mechanism, and Applications (2003, background): “Recently, two independent groups identified additional components of the RISC complex.”
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper (2017, background): “or fragile Xmental retardation syndrome-related protein 1 (FXR1) [133] play a role in selecting miRNAs for EV export.”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “Previous studies have identified a variety of different proteins as potential components of the RISC, although few of them have been functionally characterized at the molecular level ( Caudy et al., 2001 ).”
Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. (2004, background): “In Drosophila, it has been shown that Argonaute2 (AGO2) protein, a member of the Argonaute family of proteins (Carmell et al. 2002), is essential for RNAi driven by exogenously introduced dsRNA and is the first protein component to be identified as part of the RISC complex in cultured Drosophila S2…”
A mammalian microRNA expression atlas based on small RNA library sequencing. (2007, background): “…to evaluate the role of Fragile X mental retardation protein (FMRP), which was shown to interact with DICER and AGO protein in the miRNA pathway (Caudy et al., 2002; Jin et al., 2004; Plante et al., 2006), the miRNA profile of the whole brain of 4-day-old FMRP knockout mice and homozygous…”