Post-transcriptional gene silencing by double-stranded RNA

By Scott M. Hammond, Amy A. Caudy, Gregory J. Hannon. Nature reviews genetics, 2001

875 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/ad89f2446be164cc7fbddc4744c110f1d533f864

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Citations in the corpus

Transformation of Plants with Multiple Cassettes Generates Simple Transgene Integration Patterns and High Expression Levels (2005, background): “This produces complex, multicopy transgenic loci that have the potential to form DNA secondary structures and/or to express hairpin RNAs, both of which also promote transgene silencing (Plasterk and Ketting 2000; Hammond et al. 2001 ).”
RNA Interference: Biology, Mechanism, and Applications (2003, background): “melanogaster for processing the micro-RNAs (86, 95).”
ATP requirements and small interfering RNA structure in the RNA interference pathway. (2001, neutral): “the target-cleaving nuclease (Hammond et al., 2001a).”
The biosafety of molecular farming in plants (2003, background): “…are undesirable because they tend to be unstable, and in many cases contain inverted repeats or truncated transgenes that have the potential to form DNA secondary structures or to express hairpin RNAs, both of which can trigger transgene silencing (Plasterk and Ketting, 2000; Hammond et al., 2001).”
siRNA function in RNAi: a chemical modification analysis. (2003, background): “…by which specific mRNAs are targeted for degradation by complementary short-interfering RNAs (siRNAs), has increasingly become a powerful tool for genetic analysis and is likely to become a potent therapeutic approach for gene silencing (for review, see Hammond et al. 2001; McManus and Sharp 2002).”
An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. (2003, methodology): “Post-transcriptional gene silencing is a nucleotide sequence-specific RNA turnover mechanism that is highly conserved among most, if not all, eukaryotes (Hammond et al., 2001).”
Genetic transformation technology: Status and problems (2005, background): “Gene silencing is the phenomenon of nonexpression/minimal expression of a transgene and/or a homologous gene in a transgenic plant (organism) (Hammond et al., 2001).”
siRNA vs. shRNA: similarities and differences. (2009, background): “Dicer is a multi-domain RNase III-related endonuclease responsible for processing dsRNA to siRNAs [71].”
Fragile X-related protein and VIG associate with the RNA interference machinery. (2002, methodology): “Biochemical purification of the RNAi effector nuclease, RISC, revealed the Drosophila Argonaute-2 (Ago-2) protein as a core component of this complex (Hammond et al. 2001a).”
Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat (2003, background): “The high expression of antisense transcript might then be followed by formation of double-stranded RNA and subsequent post-transcriptional gene silencin”