An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.

By Olivier Voinnet, S. Rivas, P. Mestre, David C. Baulcombe. The Plant Journal, 2003

1,838 citations recorded by the source corpus.

Subfields: Biotechnology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/af14d5ffad91160ed591050f31f2bd9a8d4ab740

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Citations in the corpus

Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “Transient expression procedure Agrobacterium tumefaciens C58C1 containing p19 (Voinnet et al., 2003) and EHA105 containing the different constructions were grown at 28 °C in LB medium supplemented with 50 mg l–1 kanamycin, 50 mg l–1 rifampicin, and 100 mg l–1 gentamicin (only for EHA105) up to the s”
Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice (2009, positive): “Cultures were mixed 1:1 with similarly prepared cultures containing the construct 35S:p19, to provide co-expression of the tomato bushy stunt virus p19 protein which suppressed gene silencing (Voinnet et al., 2003).”
Approaches to Minimize Variation of Transgene Expression in Plants (2005, background): “Heterologous protein production was enhanced 50-fold or more in the presence of p19 due to prevention of PTGS in the infiltrated tissues (Voinnet et al. 2003).”
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. (2004, methodology): “For infiltration of N. benthamiana, the Agrobacterium tumefaciens strain C58C1 carrying the pCH32 helper plasmid was infiltrated into the abaxial air space of 2–4-week-old plants as described (Voinnet et al., 2003; Witte et al., 2004).”
Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10 (2007, methodology): “Infiltrations were performed according to the methods described by Voinnet et al. (2003).”