ATP requirements and small interfering RNA structure in the RNA interference pathway.

By A. Nykänen, B. Haley, Phillip D. Zamore. Cell, 2001

1,112 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2cc366b3324426b29110c67e07f94993fbcb2982

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Citations in the corpus

Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference. (2004, background): “Active RISC includes siRNA antisense strands (AS) but not sense strands (SS) (16), thus indicating that double-stranded siRNA undergoes denaturation via helicase (8) either prior to or at an early stage in RISC formation.”
Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. (2004, background): “Target mRNAs containing sequences imperfectly complementary to the miRNA can be subject to translational repression without altering mRNA stability (Lee et al. 1993; Wightman et al. 1993; Olsen and Ambros 1999; Reinhart et al. 2000; Bartel 2004).”
Gene silencing in mammals by small interfering RNAs (2002, background): “Notably, in both cases, the transcribed RNAs contain 5′ triphosphate termini, as opposed to a 5′ monophosphate that is required for siRNA functionality in Drosophila”
Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells (2002, background): “Synthetic and endogenous siRNAs direct targeted mRNA degradatio”
Small RNA sorting: matchmaking for Argonautes (2010, background): “RISC loading is an active process that requires AT”
Killing the messenger: short RNAs that silence gene expression (2003, background): “There is a strict requirement for the siRNA to be 5′ phosphorylated to enter into RIS”
An overview of microRNAs (2015, background): “These miRNA duplexes rely on strand unwinding, and several helicases have been described to possess this activity[63-67].”
siRNA-mediated gene silencing in vitro and in vivo (2002, background): “1A , pmCMVsiGFPmpA; transcript referred to as siGFP) (Experimental Protocol), because we reasoned that functional siRNA would require minimal to no overhang”
The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes (2003, background): “For RISC, this is probably accomplished by a series of coupled assembly steps that result in the generation of an active RISC comple”