Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.

By Thomas D. Schmittgen, B. A. Zakrajsek. Journal of Biochemical and Biophysical Methods, 2000

1,312 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/9b62f101c262de335618286018d9a4266cdc7a32

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Citations in the corpus

A method for the absolute quantification of cDNA using real-time PCR. (2003, background): “Whilst this is significantly superior to others, arguments can still be made to its validity (Schmittgen and Zakrajsek, 2000).”
Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction (2005, methodology): “We used the geometric mean of three control genes (Actin, G3PDH, and 18S rRNA) for accurate normalization (Vandesompele et al., 2002) to minimize errors from potential variance in a single control gene (Schmittgen and Zakrajsek, 2000; Suzuki et al., 2000).”
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. (2005, background): “Many studies on housekeeping gene expression mainly deal with human tissues (Thellin et al., 1999; Schmittgen and Zakrajsek, 2000; Warrington et al., 2000; Radonic et al., 2004), bacteria and viruses (Stöcher et al., 2002, 2003; Savli et al., 2003), and, as far as is known, only a few have…”
Quantitative real-time RT-PCR--a perspective. (2005, background): “…against one of the rRNAs (Bhatia et al. 1994, Zhong & Simons 1999). rRNA levels may vary less under conditions that a ﬀ ect the expression of mRNAs (Schmittgen & Zakrajsek 2000) and the use of rRNA has been claimed to be more reliable than that of several reference genes in rat livers (de Leeuw…”
Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR (2009, background): “Recent reports have demonstrated that some of the most well-known and frequently used reference genes are inappropriate for normalization in qPCR analysis due to expression variability (Czechowski et al. 2005; Remans et al. 2008; Schmittgen and Zakrajsek 2000 ).”
Analyzing real-time PCR data by the comparative CT method (2008, methodology): “Methodology to validate internal control genes has been described previously in ref”
Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. (2002, background): “Preferably, its steady-state expression levels should be similar to those of the target gene [5].”
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations (2004, background): “On the other hand, some of these genes has been reported to be regulated occasionally (Foss et al. 1998, Schmittgen & Zakrajsek 2000).”
Guideline to reference gene selection for quantitative real-time PCR. (2004, result): “18S rRNA is often used as a reference and has been described as a preferable control [13,14,22,23], although it has been shown to be regulated in other studies [11,24].”
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. (2002, result): “…29, 23–39 Downloaded from Bioscientifica.com at 08/22/2022 08:38:45PM via free access recently by a study that showed its levels remaining constant during serum-stimulation of tissue culture cells (Schmittgen & Zakrajsek 2000), a conclusion supported by another recent study (Goidin et al. 2001).”
Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators (2011, methodology): “An ideal reference gene, known as an internal control gene, should be expressed at a constant level across various conditions, such as developmental stages or tissue types, and its expression should be unaffected by experimental parameters (Schmittgen and Zakrajsek 2000; Thellin et al. 1999).”
Reference Gene Selection for Real-Time Quantitative Polymerase Chain Reaction of mRNA Transcript Levels in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) (2010, background): “However, numerous studies have shown that the expressions of these genes vary under experimental conditions (Guénin et al. 2009; Schmittgen and Zakrajsek 2000; Zhong and Simons 1999).”