Guideline to reference gene selection for quantitative real-time PCR.

By Aleksandar Radonic, Stefanie Thulke, I. Mackay, O. Landt, W. Siegert, Andreas Nitsche. Biochemical and Biophysical Research Communications - BBRC, 2004

1,700 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2150d9f418550864855628019bb0052b70098951

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In Molecular Biology

1. W. Scheible
2. Mark Stitt
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4. Lucien Hoffmann
5. Jiani Qi
6. Michael K. Udvardi
7. Xiuyun Zhao
8. Xiangqun Shen

Citations in the corpus

• Validation of Candidate Reference Genes for the Accurate Normalization of Real-Time Quantitative RT-PCR Data in Rice During Seed Development (2010, background): “Nevertheless, different studies sometimes gave different or even opposing opinions of these classic housekeeping genes, and it was implied that the expression of these genes actually vary under different experimental paradigms (Czechowski et al. 2005; Lee et al. 2002; Radonic et al. 2004; Vandesom”
• Standard methods for molecular research in Apis mellifera (2013, background): “The problem is that it is impossible to prove categorically that the expression of any candidate ‘invariant’ gene is not affected by the expression of the target gene (Radonić et al., 2004).”
• Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis1[w] (2005, background): “…dehydrogenase (GAPDH), elongation factor-1a (EF-1a), polyubiquitin (UBQ), actin (ACT), and a-tubulin and b-tubulin (TUA and TUB, respectively) genes (Goidin et al., 2001; Bustin, 2002; Kim et al., 2003; Andersen et al., 2004; Brunner et al., 2004; Dheda et al., 2004; Radonić et al., 2004).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The reference gene-based normalization corrects for variable starting amounts of RNA and for differences in reverse transcription efficiency, as the references are exposed to the same preparation steps as the gene of interest (Radonić et al., 2004; Bustin, 2005; Huggett et al., 2005; Udvardi et al.,”
• Evidence that TMPRSS2 Activates the Severe Acute Respiratory Syndrome Coronavirus Spike Protein for Membrane Fusion and Reduces Viral Control by the Humoral Immune Response (2011, background): “respective CT value for the TATA-box binding protein (housekeeping reference gene) (50).”
• Reference genes identified in the silkworm Bombyx mori during metamorphism based on oligonucleotide microarray and confirmed by qRT‐PCR (2008, methodology): “Several methods, including northern blotting, quantitative real-time polymerase chain reaction (qRT-PCR), microarray and so on, are widely used to quantify gene expression levels (Radonic et al., 2004; Thellin et al., 1999).”
• Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon (2005, methodology): “A large number of genes have for this reason been selected for normalization of mRNA expression data [2, 3 ].”
• Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy (2012, methodology): “3) were compared by the quantitation of TATA Binding Protein RNA [23] and the quantitation of total HIV DNA, and showed no trend towards higher values in the post-VOR samples.”
• Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. (2005, background): “…on housekeeping gene expression mainly deal with human tissues (Thellin et al., 1999; Schmittgen and Zakrajsek, 2000; Warrington et al., 2000; Radonic et al., 2004), bacteria and viruses (Stöcher et al., 2002, 2003; Savli et al., 2003), and, as far as is known, only a few have concerned…”
• Immunostimulatory effect of Aloe vera (Aloe barbadensis) on non-specific immune response, immune gene expression, and experimental challenge with Saprolegnia parasitica in rainbow trout (Oncorhynchus mykiss) (2019, positive): “PCR efficiency was calculated using the following equation: E%=10 − 1×100 (Radonic et al., 2004).”
• Reference Gene Selection for Real-Time Quantitative Polymerase Chain Reaction of mRNA Transcript Levels in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) (2010, background): “Studies on the expression of housekeeping genes have mainly been performed on human tissues (Thellin et al. 1999; Schmittgen and Zakrajsek 2000; Warrington et al. 2000; Radonic et al. 2004), bacteria, and viruses (Stöcher et al. 2002, 2003; Savli et al. 2003).”