Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies.

By C. Tricarico, P. Pinzani, Simonetta Bianchi, Milena Paglierani, Vito Distante, Mario Pazzagli, Stephen A. Bustin, Claudio Orlando. Analytical Biochemistry, 2002

599 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7739013c2d372cf99b573a123c93c4606839c485

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Citations in the corpus

Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators (2011, methodology): “As a consequence, the normalization of expression data obtained by employing more advanced methods of gene expression study, such as real-time PCR or microarrays, results in the subsequent misinterpretation of the results (Schmittgen and Zakrajsek 2000; Tricarico et al. 2002).”
Normalization of microRNA expression levels in quantitative RT-PCR assays: identification of suitable reference RNA targets in normal and cancerous human solid tissues. (2008, methodology): “The accuracy of these methods for expression analysis, however, is critically dependent on proper normalization of the data inasmuch as inappropriate normalization of qRT-PCR data can lead to incorrect conclusions (Tricarico et al. 2002; Bas et al. 2004).”
Differential gene expression in diapausing and active Calanus finmarchicus (Copepoda) (2008, methodology): “(3) Another approach is to normalize samples to the starting amounts of total RNA or cDNA (Tricarico et al. 2002).”
Quantitative real-time RT-PCR--a perspective. (2005, background): “…model identifying GAPDH as one of the most suitable reference genes in colorectal cancer, when there is considerable evidence that mRNA levels of this gene vary signiﬁcantly between individuals and between paired normal and cancer tissue (Bustin et al. 1999, Bustin 2000, Tricarico et al. 2002).”
Guideline to reference gene selection for quantitative real-time PCR. (2004, background): “However, it has been reported previously that TBP is a highly regulated gene when comparing normal and tumor tissues from breast cancer biopsies [15].”
GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. (2005, background): “Consequently, individually these genes are generally unsuitable as expression controls, and if they are used for normalizations across different tissues, significant errors can be introduced (3, 11, 13).”
Quantification of mRNA using real-time RT-PCR (2006, background): “The inevitable consequence of inappropriate normalization is the acquisition of biologically irrelevant dat”
Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing (2006, background): “There is no single housekeeping gene that is suitable for all experimental conditions and articles have been published on the variability of certain housekeeping genes in different systems (326, 498).”