Analyzing real-time PCR data by the comparative CT method
By Thomas D. Schmittgen, K. Livak. Nature Protocols, 2008
25,209 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/4580ba914fe16fae7d733b96e37a282bed953437
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In Molecular Biology
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Citations in the corpus
- Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil? (2017, methodology): “(2)) was used to calculate the fold change of each ARG (FC value) in treatments compared to the control (Schmittgen and Livak, 2008).”
- Identification and validation of conserved microRNAs along with their differential expression in roots of Vigna unguiculata grown under salt stress (2010, methodology): “Relative expression levels for each sample were obtained using the ‘‘comparative Ct method’’ (Schmittgen and Livak 2008).”
- Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Expression of AhNRAMP1 , as well as OsNRAMP1 , significantly improved yeast growth compared with the control strain transformed with empty vector under various pH values tested (pH 5, 6, and 7) (Fig.”
- Repurposing the CRISPR-Cas9 system for targeted DNA methylation (2016, methodology): “The expression of the target genes BACH2 and IL6ST was normalized to the reference gene HPRT1 and analyzed using the comparative Cq method (38).”
- Paternal stress exposure alters sperm microRNA content and reprograms offspring HPA stress axis regulation (2013, methodology): “Analysis was performed using the comparative Ct method (Schmittgen and Livak, 2008).”
- Constitutive AP-1 Activity and EBV Infection Induce PD-L1 in Hodgkin Lymphomas and Post-transplant Lymphoproliferative Disorders: Implications for Targeted Therapy (2012, methodology): “Relative gene transcript levels were calculated by the comparative CT method (28).”
- The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. (2009, methodology): “CVs should not be used with Cqs, however ( 29 ).”