Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction

By Sheng Zhao, Russell D. Fernald. J. Comput. Biol., 2005

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Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR (2009, methodology): “…total volume of 20 ll. Reaction mixtures were incubated for 5 min at 94 C, followed by 40 amplification cycles of 15 s at 94 C, 10 s at 60 C, and 15 s at 72 C. PCR efficiencies and optimal cycle threshold (Ct) values were estimated using the online real-time PCR Miner tool (Zhao and Fernald 2005).”
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data (2009, methodology): “It has been addressed in some papers (7,21) and then it is mainly discussed in the context of the fit of the employed analysis model (5,7).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “…and drives AhNRAMP1 cDNA under the control of the –272/–131 region of the barley IDS2 gene containing Fe-deﬁciency-responsive element 1 (IDE1) and IDE2, ﬂanked by the –90/+8 region of the CaMV35S X tobacco ( Nicotiana tabacum L. cv. Petit-Havana SR1) according to the method of Helmer et al. (1984).”