Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.

By N. Nicot, J. Hausman, Lucien Hoffmann, D. Evers. Journal of Experimental Botany, 2005

1,310 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/e00c96d4aa691dabd097c9a20b25e372e03ca778

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Citations in the corpus

Identification of Four Soybean Reference Genes for Gene Expression Normalization (2008, background): “In plants, EF1-α in potato (Solanum tuberosum L.; Nicot et al., 2005), GAPDH, actin and EF1-α in grape (Vitis spp.”
Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. (2006, methodology): “Recently, the validation of seven housekeeping genes using geNorm demonstrated that the ef1a is most stably expressed during biotic and abiotic stresses in potato [28].”
Reference Gene Selection for Real-Time Quantitative Polymerase Chain Reaction of mRNA Transcript Levels in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) (2010, methodology): “ Nicot et al. (2005) showed that EF-1-α was the most stable gene in potato under abiotic stress (salt and cold stress) of potato, but our work revealed that the expression level of this gene was not stable in the Chinese cabbage”
Enhancement of artemisinin content and biomass in Artemisia annua by exogenous GA3 treatment (2010, background): “The internal reference gene in this experiment was Elongation factor 1-alpha (EF1-a), which was previously reported to be the stable housekeeping gene under biotic and abiotic stresses (Nicot et al. 2005).”
Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators (2011, background): “…different experimental systems the expression of the commonly used reference genes 18S rRNA, ACTB, TUA or GAPDH often remains unstable (Barber et al. 2005; Bas et al. 2004; Dheda et al. 2004; Jemiolo and Trappe 2004; Nicot et al. 2005; Remans et al. 2008; Yperman et al. 2004; Zhaoguo et al. 2009).”
An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development (2006, neutral): “Examples such as GAPDH, 18S rRNA and EF1-α have been widely used in RNA blot analyses and are commonly used for real-time RT-PCR in various plant species [2,3,6,7,10,11].”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The potato elongation factor 1α (Ef-1α) was used as a reference gene (Nicot et al., 2005; Orłowska et al., 2012).”
Validation of Candidate Reference Genes for the Accurate Normalization of Real-Time Quantitative RT-PCR Data in Rice During Seed Development (2010, background): “It is thus important and necessary to select suitable reference gene(s) for different species according to different experimental and developmental conditions ( Nicot et al. 2005 ; R obinson et al.2007; Gutierrez et al. 2008).”
Reference genes identified in the silkworm Bombyx mori during metamorphism based on oligonucleotide microarray and confirmed by qRT‐PCR (2008, background): “Translation initiation factors and elongation factors often have been identified as housekeeping genes and are widely used as reference genes for gene expression measurement (Eisenberg & Levanon, 2003; Nicot et al., 2005).”
Reference genes in real-time PCR (2013, methodology): “Focusing on salinity stress and difficulties in water extraction from the soil—for three different plants: potato (Nicot et al. 2005), Lolium temulentum L. (Dombrowski and Martin 2009) and cucumber (Wan et al. 2010), ACT gene was ranked in the last place in the ranking of stability every time,…”