A method for the absolute quantification of cDNA using real-time PCR.

By J. Whelan, N. Russell, M. Whelan. JIM - Journal of Immunological Methods, 2003

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Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots (2011, methodology): “The concentration of plasmid DNA was measured and converted to copy concentration using the following equation as described by Whelan et al. (2003): DNA copyð Þ ¼ 6:02 10 23 copies mol 1 DNA amount gð Þ DNA length bpð Þ 660 gmol 1 bp 1 : Tenfold dilutions were carried out and used as external…”
Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing (2014, methodology): “The number of 16S rDNA copies present in the plasmid preparation was estimated based on the DNA concentration and molecular mass of pGEM-T Easy with the target insert, as previously described [29].”