Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome Organization

By Hirotami Matsuo, J. Chevallier, N. Mayran, I. Le Blanc, C. Ferguson, J. Fauré, N. S. Blanc, S. Matile, J. Dubochet, R. Sadoul and 3 more. Science, 2004

705 citations recorded by the source corpus.

What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/956e82b705f3343450cf3f1d99fb9e644c1de808

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Citations in the corpus

Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, background): “CD63 is instrumental in targeting the EBV-encoded LMP1 protein to ILVs and allowing its subsequent release in exosomes (Verweij et al. 2011).”
How proteins produce cellular membrane curvature (2006, background): “An example might be the presence of large membrane concentrations of an unconventional phospholipid, lysobisphosphatidic acid (LBPA), which is present in late endosomes and has been shown to generate, in conjunction with transmembrane pH gradients, multivesicular liposome”
T cell receptor-proximal signals are sustained in peripheral microclusters and terminated in the central supramolecular activation cluster. (2006, background): “TCR degradation takes place via ubiquitination and targeting to multivesicular bodies that are enriched in lysobisphosphatidic acid (LBPA) in the lysosomal pathway (Liu et al., 2000; Matsuo et al., 2004).”
Exosomes are released by cultured cortical neurones. (2006, background): “We recently showed that MVB biogenesis is severely impaired in HeLa cells depleted of Alix by siRNA (Matsuo et al., 2004); and Tsg101, which interacts with Alix, is a known component of the so-called endosomal sorting complex required for transport (ESCRT) necessary for the sorting of ubiquitinated…”
Syndecan–syntenin–ALIX regulates the biogenesis of exosomes (2012, background): “Note that truncated forms of Alix containing the V domain interact with the two parts of the N-Ter domain of syntenin that contain LYPXL motifs, (1-30) and (31-60), and that mutation of the three LYPXL motifs (YP to AA) abolishes the interaction.”
Classification, Functions, and Clinical Relevance of Extracellular Vesicles (2012, background): “Further evidence for two pathways comes from studies showing that lysobisphosphatidic acid induces the formation of intraluminal vesicles predestined for lysosomal degradation but does not affect the formation of exosomes (Matsuo et al., 2004).”
Cellular cholesterol trafficking and compartmentalization (2008, background): “LBPA is structurally important for formation of the highly curved internal membranes of multivesicular bodie”