LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing

By Y. Kabeya, N. Mizushima, T. Ueno, A. Yamamoto, T. Kirisako, Takeshi Noda, E. Kominami, Y. Ohsumi, T. Yoshimori. EMBO Journal, 2000

6,838 citations recorded by the source corpus.

Subfields: Epidemiology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/93c757169dcebffd1ae6798dc5ea824ec0736c0c

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Citations in the corpus

PI(5)P Regulates Autophagosome Biogenesis (2015, background): “…2015 ª2015 The Authors 219 (legend on next page) 220 Molecular Cell 57, 219–234, January 22, 2015 ª2015 The Authors versus a loading control (e.g., tubulin), or by scoring the number of LC3-positive vesicles, since LC3-II is specifically targeted to autophagosomal membranes (Kabeya et al., 2000).”
Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG (2006, background): “During autophagy, the LC3 precursor (LC3-I) undergoes cleavage and lipidation to a processed form (LC3-II”
Autophagy Is Activated for Cell Survival after Endoplasmic ReticulumStress (2006, methodology): “Next, GFP-LC3 fluorescence was used to monitor autophagy in cells transiently transfected with an expression vector for GFP-LC3 (8).”
Regulation Mechanisms and Signaling Pathways of Autophagy (2009, background): “In nutrient-rich conditions, the majority of Atg8 is cytosolic; upon autophagy induction, Atg8 largely exists as the lipid-conjugated form and is localized to both sides of the phagophore (58,65).”
Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages (2009, methodology): “We analysed autophagy in these cells by morphological observation of GFP–LC3, a marker of autophagosome”
Functional and physical interaction between Bcl‐XL and a BH3‐like domain in Beclin‐1 (2007, methodology): “Cells were transfected with empty vector alone or together with LC3-GFP (Kabeya et al, 2000), in the presence or absence of Beclin-1 wild type, Beclin-1 L116A, Beclin-1 F123A, Bcl-XL wild type, Bcl-XL G138A, Bcl-2 wild type, or Mcl-1.”
Molecular definitions of autophagy and related processes (2017, neutral): “Lipidated LC3 (often referred to as LC3-II) is generated onto forming autophagosomes and allows for substrate uptake upon binding to several autophagy receptors (Kabeya et al , 2000; Stolz et al , 2014; Wild et al , 2014).”
Protein turnover via autophagy: implications for metabolism. (2007, background): “The two forms of LC3, LC3-I and LC3-II, are easily separated by SDS-PAGE; LC3II migrates faster than LC3-I because of its extreme hydrophobicity (33).”
The Atg8 and Atg12 ubiquitin‐like conjugation systems in macroautophagy (2008, background): “J Biol Chem 275: 5845–5851 ichimura y et al (2000) a ubiquitin-like system mediates protein lipidation.”
Glutaminolysis activates Rag-mTORC1 signaling. (2012, background): “U2OS cells were plated on coverslips and transfected with a GFP-LC3 overexpressing plasmid.”