Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain

By Jinbiao Ma, K. Ye, Dinshaw J. Patel. Nature, 2004

781 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/1f82ba45ad724cf763e05a1a0691644e82e1caef

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In Molecular Biology

Citations in the corpus

Human MicroRNA Targets (2004, background): “A first step in this direction is the very recent analysis of the crystal structure of a PAZ domain of a human Argonaute protein, eIF2c1, complexed with a 9- mer RNA oligonucleotide in dimer configuration, which may represent three-dimensional interactions for the 39 end of a miRNA (and siRNA) compl”
MicroRNA regulation by RNA-binding proteins and its implications for cancer (2011, background): “The 5′ phosphate group of the miRNA guide strand is stably bound by the MID domain while the PAZ domain recognizes the dinucleotide 3′ overhang that is characteristic of Dicer-mediated cleavag”
Strategies for silencing human disease using RNA interference (2007, background): “and a PIWI–Argonaute–Zwille (PAZ) domain that recognizes the siRNA 3′ en”
Mechanisms of gene silencing by double-stranded RNA (2004, background): “Recent biochemical and structural studies, however, converged on the view that PAZ is an RBD that specifically recognizes the terminus of the base-paired helix of siRNA and miRNA duplexes, including the characteristic 2-nucleotide 3′ overhang”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, background): “This is then followed by the association of the PAZ domain (Lingel et al., 2003; Song et al., 2003; Ma et al., 2004; Yan et al., 2004) of Ago2 with an ensuing single-stranded siRNA.”
How do microRNAs regulate gene expression? (2008, background): “Cocrystal structures show that the 5′-phosphate on the siRNA is essential to place this phosphodiester bond in the active site (16, 17 ).”
Perspective: machines for RNAi. (2005, background): “The PAZ motif is thought to bind single stranded RNA (Lingel et al. 2003; Song et al. 2003; Yan et al. 2003; Lingel et al. 2004; Ma et al. 2004).”
The Structure of Human Argonaute-2 in Complex with miR-20a (2012, positive): “This was confirmed by studies of the isolated PAZ (P-element induced wimpy testes [PIWI], Argonaute and Zwille) domain, whose structure revealed an oligonucleotide-binding fold that engaged the 30 ends of small RNA guides (Lingel et al., 2003, 2004; Ma et al., 2004; Song et al., 2003; Yan et al., 20”