The tandem affinity purification (TAP) method: a general procedure of protein complex purification.

By O. Puig, F. Caspary, G. Rigaut, B. Rutz, E. Bouveret, E. Bragado‐Nilsson, M. Wilm, Bertrand Séraphin. Methods, 2001

1,935 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/90a5e001106cf4b8ea310ede4134ab7b9716dae1

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Citations in the corpus

Deciphering Protein–Protein Interactions. Part I. Experimental Techniques and Databases (2007, methodology): “A TAP tag consists of two IgG binding domains of Staphylococcus protein A and a calmodulin binding peptide separated by the tobacco etch virus protease cleavage site [61,62] (Figure 1C).”
Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription. (2005, methodology): “To gain a complete understanding of the subunits that constitute each of these complexes, whole cell extract from a tandem affinity purification (TAP) (Puig et al., 2001) tagged Rpd3 strain was subjected to a modified TAP purification that resolves the two Rpd3 complexes on a MonoQ ion-exchange colu”
Understanding storage starch biosynthesis in plants: a means to quality improvement (2006, background): “Rapid progress has been made in our understanding of a variety of protein complexes in yeast cells using tandem affinity purification (TAP) of tagged recombinant proteins in conjunction with mass spectrometry (MS) (Rigaut et al. 1999; Puig et al. 2001).”
Analysis of Genes and Genomes (2004, background): “These elements are separated by a TEV protease cleavage site (Puig et al., 2001).”
Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants1[C][W][OA] (2007, methodology): “Tandem affinity purification represents a more advanced technique primarily designed to identify new proteins in a protein complex in a native state (Puig et al., 2001; Rohila et al., 2006).”
Yeast Two-Hybrid, a Powerful Tool for Systems Biology (2009, positive): “As compared to the single Flag-tag approach, combination of two different purification steps in TAP results in improved sensitivity and specificity (TAP is reviewed in more detail in [39,40]).”
Structural and Functional Conservation of the NuA4 Histone Acetyltransferase Complex from Yeast to Humans (2004, methodology): “for nucleosomal HAT activity, full-length Tip60, Tip60b, ING3, ING3(1-300),”
The AP2/ERF Domain Transcription Factor ORA59 Integrates Jasmonic Acid and Ethylene Signals in Plant Defense1[W] (2008, methodology): “The XVE-ORA59-TAP and XVE-ERF1-TAP lines carry an inducible expression module of the ORA59 and ERF1 genes, respectively, fused to a TAP tag (Puig et al., 2001).”
The IntAct molecular interaction database in 2010 (2009, background): “Others, for example X-ray crystallography or tandem affinity purification (2), typically provide information on associations of more than two proteins.”
Pre-mRNA splicing: awash in a sea of proteins. (2003, methodology): “(2002) employed the TAP-tag (for Tandem Affinity Purification), only 82 integral proteins (although it should be noted that ribosomes have yet to be subjected to comprehensive originally developed by Seraphin and coworkers (Puig et al., 2001).”
ChIP-chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments. (2004, methodology): “DNA fragments crosslinked to the protein of interest are enriched in one of three standard ways: immunoprecipitation with a protein-specific antibody, immunoprecipitation of a tagged protein using an antibody specific to the tag, or affinity purification using a tag that obviates the need for antibo”
Functional organization of the yeast proteome by systematic analysis of protein complexes (2002, methodology): “This technique combines a first high-affinity purification, mild elution using a site-specific protease, and a second affinity purification to obtain protein complexes with high efficiency and specificit”
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells (2009, methodology): “For tandem affinity purification (TAP) experiments, we created Entry vectors encoding tandem N-terminal epitope tags in three reading frames, based on the pNTAP vector from Stratagene (NTAP,[28]).”