Deciphering Protein–Protein Interactions. Part I. Experimental Techniques and Databases

By Benjamin A. Shoemaker, Anna R. Panchenko. PLoS Comput. Biol., 2007

380 citations recorded by the source corpus.

Proteins interact with each other in a highly specific manner, and protein interactions play a key role in many cellular processes; in particular, the distortion of protein interfaces may lead to the development of many diseases. To understand the mechanisms of protein recognition at the molecular level and to unravel the global picture of protein interactions in the cell, different experimental techniques have been developed. Some methods characterize individual protein interactions while others are advanced for screening interactions on a genome-wide scale. In this review we describe different experimental techniques of protein interaction identification together with various databases which attempt to classify the large array of experimental data. We discuss the main promises and pitfalls of different methods and present several approaches to verify and validate the diverse experimental data produced by high-throughput techniques.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a6f32356c6b6451d78265c1c04f7a3d1ab700b92

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In Molecular Biology

Citations in the corpus

iRefIndex: A consolidated protein interaction database with provenance (2008, methodology): “These data are generated by a multitude of methods including both high-throughput and more traditional low-through-put proteomics studies [1] as well as in silico predictions based on known interactions [2].”
Semi-supervised multi-task learning for predicting interactions between HIV-1 and human proteins (2010, background): “Meanwhile, there exists a considerable amount of protein pairs where an association appears between two partners, but not enough experimental evidence to support it as a direct interaction (partially labeled).”
Deciphering Protein–Protein Interactions. Part II. Computational Methods to Predict Protein and Domain Interaction Partners (2007, background): “In our companion review published in the last issue [”
Protein–Protein Interactions Essentials: Key Concepts to Building and Analyzing Interactome Networks (2010, background): “Several previous publications describe databases related to protein interactions [13–15].”