Channelrhodopsin-2–assisted circuit mapping of long-range callosal projections

By Leopoldo T. Petreanu, Daniel Huber, A. Sobczyk, Karel Svoboda. Nature Neuroscience, 2007

920 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/7504e8db794e20d9aee8ebfee1df28d5ccaeb2c4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

In Cognitive Neuroscience

Citations in the corpus

Optogenetic investigation of neural circuits underlying brain disease in animal models (2012, background): “, several elegant optogenetic studies have already made substantial advances in detailed circuit mappin”
Spatiotemporal dynamics of cortical sensorimotor integration in behaving mice. (2007, result): “The paucity of callosal axons terminating in the barrel cortex of the opposite hemisphere is also in good agreement with recent mouse data showing that only a small very lateral portion of the barrel field is innervated by supragranular callosal axons (Petreanu et al., 2007).”
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators (2009, methodology): “L2/3 progenitor cells were transfected via in utero electroporation in C57BL/6Crl pregnant mice at E16 with a plasmid expressing CRE recombinase under the CAGS promoter as previously describe”
Diaschisis: past, present, future. (2014, background): “sin ChR2 in slice (Petreanu et al., 2007) and in living animals (Lim”
The functional organization of the barrel cortex. (2007, background): “Callosal connectivity between the barrel cortices on opposite hemispheres appears to be limited to the representation of the most medial A-row whiskers (Petreanu et al., 2007).”
Closed-loop and activity-guided optogenetic control. (2015, positive): “…developmental stage (e.g., birth date) as in the case of in utero electroporation to target specific layers in cortex (Saito and Nakatsuji, 2001; Gradinaru et al., 2007; Petreanu et al., 2007) and by promoter-based strategies and viral tropism (Adamantidis et al., 2007; Nathanson et al., 2009).”
A Sensorimotor Circuit in Mouse Cortex for Visual Flow Predictions. (2017, neutral): “(F) Left: Injection scheme for CRACM.”
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, background): “…non-overlapping imaging/optogenetics will enable qualitatively new sorts of neuroscience experiments, such as mapping projections of numerous single neurons through brain tissue, akin to an “all-optical” channelrhodopsin-assisted circuit mapping (CRACM) experiment (Petreanu et al., 2007).”
A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing (2012, methodology): “ChR2(H134R)-tdTomato was synthesized based on the hChR2 sequence 26 , and ChR2(H134R)-EYFP was synthesized based on the ChR2 sequence 3 .”
Genetic dissection of neural circuits. (2008, methodology): “Remarkably, even ChR2-positive axons that are severed from their parent somatacanbephotostimulated to fire actionpotentials (Petreanu et al., 2007).”
The Development and Application of Optogenetics (2011, methodology): “Optogenetic tools have been well tolerated when electroporated in utero into mouse embryos (Adesnik & Scanziani 2010, Gradinaru et al. 2007, Lewis et al. 2009, Petreanu et al. 2007); IUE may www.annualreviews.org • The Development and Application of Optogenetics 399 A nn u.”
Molecular and cellular approaches for diversifying and extending optogenetics. (2010, background): “…species such as rats and primates), as with earlier projection-based targeting methods that are not transcellular (Gradinaru et al., 2007, 2009; Petreanu et al., 2007, 2009; Lima et al., 2009); but, if needed, such additional genetic refinements can be readily added (for example, both the…”