Molecular and cellular approaches for diversifying and extending optogenetics.

By Viviana Gradinaru, Feng Zhang, Charu Ramakrishnan, J. Mattis, R. Prakash, Ilka Diester, I. Goshen, K. Thompson, Karl Deisseroth. Cell, 2010

1,019 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cellular and Molecular Neuroscience, Plant Science

View Paper: https://www.semanticscholar.org/paper/d850ec86205e64574ff2d4c3e3d8242cf495bf70

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Citations in the corpus

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters (2018, neutral): “We fused Archons with EGFP for visualization, and Golgi export trafficking signal (KGC) and endoplasmic reticulum export (ER) sequences 27,28 (Fig.”
DREADDs for Neuroscientists (2016, methodology): “A key innovation for the development of AAV- and lentiviral-based FLEX switch vectors (also known as double-floxed inverse open reading frame [DIO]) (Gradinaru et al., 2010) was the use of separate antiparallel loxP-type recombination sites (especially loxP and lox2272) that allow for homotypic but ”
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance (2015, positive): “Protein engineering, such as codon optimization and addition of membrane-targeting tags (Gradinaru et al., 2010), is essential for optimizing expression in the mammalian brain.”
Optogenetic tools for analyzing the neural circuits of behavior (2011, background): “Antero-grade labeling or retrograde labeling of deﬁned projections that enter a target region, or that innervate a target cell type, is also a possibility, given speciﬁc viruses with this capability [32,67 – 69].”
Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins (2011, background): “(iv) Finally the nature of the action spectrum will dictate feasibility of combining with other light-activated reagents in the same preparatio”
Recombinase-driver rat lines: tools, techniques, and optogenetic application to dopamine-mediated reinforcement (2011, positive): “…specificity for control of cells or projections within a particular illuminated brain region arises because opsins traffic efficiently down axons (Gradinaru et al., 2010) and incoming afferents from other brain regions that are photosensitive will confound experiments by exhibiting optical…”