A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing
By L. Madisen, Tianyi Mao, H. Koch, Jia-min Zhuo, A. Berényi, Shigeyoshi Fujisawa, Yun-Wei A. Hsu, Alfredo J. Garcia, Xuan Gu, S. Zanella and 12 more. Nature Neuroscience, 2012
1,320 citations recorded by the source corpus.
Cell type–specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of four knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0 and archaerhodopsin Arch-ER2. All four transgenes mediated Cre-dependent, robust activation or silencing of cortical pyramidal neurons in vitro and in vivo upon light stimulation, with ChR2-EYFP and Arch-ER2 demonstrating light sensitivity approaching that of in utero or virally transduced neurons. We further show specific photoactivation of parvalbumin-positive interneurons in behaving ChR2-EYFP reporter mice. The robust, consistent and inducible nature of our ChR2 mice represents a significant advance over previous lines, and the Arch-ER2 and eNpHR3.0 mice are to our knowledge the first demonstration of successful conditional transgenic optogenetic silencing. When combined with the hundreds of available Cre driver lines, this optimized toolbox of reporter mice will enable widespread investigations of neural circuit function with unprecedented reliability and accuracy.
Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience
View Paper: https://www.semanticscholar.org/paper/d3b513f849a90e11c3977bff677ba6612e60a7f1
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Citations in the corpus
- Distinct Contribution of Adult-Born Hippocampal Granule Cells to Context Encoding (2016, neutral): “Male Ai9, Ai35, Nestin-CreERT2, and controls were generated as previously described (Dranovsky et al., 2011; Madisen et al., 2010, 2012).”
- Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance (2015, neutral): “0 (Madisen et al., 2010, 2012; Zariwala et al., 2012).”
- Differential control of learning and anxiety along the dorso-ventral axis of the dentate gyrus (2013, methodology): “0-YFP (Ai39) and POMC-Cre were generated as previously described (Madisen et al., 2012; McHugh et al., 2007).”
- Regional blood flow in the normal and ischemic brain is controlled by arteriolar smooth muscle cell contractility and not by capillary pericytes (2015, neutral): “The following transgenic mice were used: NG2cre (Zhu et al., 2008) (Jackson Labs number 008533), NG2creER (Zhu et al., 2011) (Jackson Labs number 008538), floxed ChR2-YFP (Madisen et al., 2012) (Jackson Labs number 012569), SMA-mCherry (Armstrong et al., 2010); SMAcreER (Wendling et al., 2009), mT/m”
- A Neural Circuit for Gut-Induced Reward. (2018, neutral): “Next, we separately transfected the right and left NG with the light-sensitive depolarizing channel Channelrhodopsin2 by injecting the Cre-inducible viral construct AAV-EF1a-DIO-hChR2(H134R)-EYFP (ChR2, Madisen et al., 2012).”
- In situ transcription profiling of single cells reveals spatial organization of cells in the mouse hippocampus (2016, positive): “…M anuscript A uthor M anuscript A uthor M anuscript Brain extraction and sample mounting C57BL/6 with Ai6 Cre-reporter (uncrossed) (Jackson Labs, SN: 007906) female mice aged 50–80 days were anesthetized with isoflurane according to institute protocols (protocol #1701-14) (Madisen et al., 2012).”
- Permanent Genetic Access to Transiently Active Neurons via TRAP: Targeted Recombination in Active Populations (2013, positive): “Fortunately, many high-efficiency transgenes identical in locus and design to the AI14 transgene used here have been developed for Cre-dependent expression of fluorescent proteins, optogenetic tools, and calcium indicators (Madisen et al., 2012; Madisen et al., 2010; Zariwala et al., 2012).”
- GLUT2, glucose sensing and glucose homeostasis (2015, background): “TheGlut2-Cremice were then crossed with Rosa26ChR2 [76] mice to express channelrhodopsin-2, a lightsensitive cation channel [77], in Glut2-expressing neurons.”
- Shared cortex-cerebellum dynamics in the execution and learning of a motor task (2019, neutral): “In addition, 12 wild-type C57/bl6 mice were used for tracing studies in Figure S1, and 3 double-transgenicGad2-Cre (Taniguchi et al., 2011) /Ai32 (CAG-lox-stop-lox-ChR2) (Madisen et al., 2012) on mixed genetic background were used for the optogenetic behavioral studies in Figure S3.”