The Development and Application of Optogenetics

By Lief Ericsson Fenno, Ofer Yizhar, Karl Deisseroth. Annual Review of Neuroscience, 2011

1,787 citations recorded by the source corpus.

Genetically encoded, single-component optogenetic tools have made a significant impact on neuroscience, enabling specific modulation of selected cells within complex neural tissues. As the optogenetic toolbox contents grow and diversify, the opportunities for neuroscience continue to grow. In this review, we outline the development of currently available single-component optogenetic tools and summarize the application of various optogenetic tools in diverse model organisms.

Subfields: Biomedical Engineering, Cellular and Molecular Neuroscience

View Paper: https://www.semanticscholar.org/paper/90406c5df9ff364e08e8dffa33a7bbd05d6868af

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. Antonio Rangel
2. John A. Clithero
3. George G. Malliaras
4. Karl Deisseroth
5. Jonathan Rivnay

In Cellular and Molecular Neuroscience

1. Lief Ericsson Fenno
2. John A. Clithero
3. Karl Deisseroth
4. Antonio Rangel
5. George G. Malliaras
6. Haruo Kasai
7. K. Tye
8. Rafael Yuste

Citations in the corpus

• Macrophages Facilitate Electrical Conduction in the Heart (2017, neutral): “To investigate cell-cell communication directly in the AV node, we expressed the photoactivatable channelrhodopsin 2 (ChR2) (Fenno et al., 2011) in macrophages to control their membrane potential.”
• Dopamine release in the basal ganglia (2011, background): “However, advances in optogenetics permit optical stimulation (or suppression) of specific cell types (Deisseroth, 2010; 2011; Zhang et al., 2010; Fenno et al., 2011; Kravitz and Kreitzer, 2011; Toettcher et al., 2011), allowing new questions about DA release to be addressed.”
• GLUT2, glucose sensing and glucose homeostasis (2015, background): “TheGlut2-Cremice were then crossed with Rosa26ChR2 [76] mice to express channelrhodopsin-2, a lightsensitive cation channel [77], in Glut2-expressing neurons.”
• Infrared light excites cells by changing their electrical capacitance (2012, methodology): “Equations (1) and (2) relate the intrinsic and transmembrane potential-induced charges on both sides of the bilayer to potentials at each bilayer surface, corrected by the Stern potential drop on each side (described by Eqs.”
• Closed-loop and activity-guided optogenetic control. (2015, neutral): “…cell types during animal behavior (in some cases at single-cell resolution in living mammals; Prakash et al., 2012; Rickgauer et al., 2014; Packer et al., 2015), is well-suited for closed-loop control in biological systems (for a history and overview of optogenetics, see Fenno et al., 2011).”
• Identifying and Quantifying Multisensory Integration: A Tutorial Review (2014, background): “…will undoubtedly shed important light on multisensory neurons and their contributions to behavior and perception is optogenetics, because of its ability to excite and inhibit specific neuron populations (Deisseroth 2011; Boyden 2011; Mattis et al. 2012; Yizhar et al. 2011a, b; Fenno et al. 2011).”