Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5′ UTR as in the 3′ UTR

By J. Lytle, T. Yario, J. Steitz. Proceedings of the National Academy of Sciences of the United States of America, 2007

1,267 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/728805de561a5b816e05032e8e0416d6d29e3da0

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In Molecular Biology

Citations in the corpus

Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? (2008, background): “miRNAs also exert their repressive function when their binding sites are artificially placed in 5′ UTRs or coding region”
miR-148 targets human DNMT3b protein coding region. (2008, background): “On the other hand, experiments with miRNA target sites cloned in the 59UTR of a luciferase construct showed miRNA-mediated repression (Lytle et al. 2007), suggesting that repression might be independent of the target site location.”
A search for conserved sequences in coding regions reveals that the let-7 microRNA targets Dicer within its coding sequence (2008, background): “More recent reports have also shown that, contrary to other highly conserved coding region motifs, miRNA target sites are conserved in all three reading frames among Drosophila genomes (11), and that target sites introduced into the 5 UTR of transcripts can repress translation (12).”
Revisiting the principles of microRNA target recognition and mode of action (2009, background): “In fact, experiments with artificial sensor constructs in human cells showed that miRNA-guided repression is equally efficient when sites are located in the 5′ or 3′ UTRs of reporter transcripts, thereby demonstrating that no mechanistic requirement confines miRNA action to 3′ UTR”
MicroRNAs in body fluids—the mix of hormones and biomarkers (2011, neutral): “This miRNA duplex is unwound into the mature single-strand form and incorporated into the RNA-induced silencing complex (RISC), which guides the complex into the complementary 3′ or 5′-untranslated region (UTR) of the target mRNA”
Regulation of mRNA translation and stability by microRNAs. (2010, background): “It is important to establish what rules apply to these noncanonical miRNA-mRNA interactions. miR-122, a liver-specific miRNA, stimulates replication of the HCV RNA in hepatoma cells by binding to the 5′ UTR upstream of the HCV RNA IRES (135).”
Origins and Mechanisms of miRNAs and siRNAs (2009, result): “Although this might suggest a UTR rule, other studies show that miRNA interaction with the 5 0 UTR can repress a postinitiation step of translation (Lytle et al., 2007).”
MicroRNA dysregulation in cancer: diagnostics, monitoring and therapeutics. A comprehensive review (2012, neutral): “Moreover, even though it is known that microRNAs mainly recognize complementary sequences in the 3 0 -untranslated regions (UTRs) of their target mRNAs, more recent studies have reported that they can also bind to the 5 0 -UTR or the ORF (Lytle et al, 2007; Moretti et al, 2010; Ørom et al, 2008; Qin”
MicroRNAs: New Biomarkers for Diagnosis, Prognosis, Therapy Prediction and Therapeutic Tools for Breast Cancer (2015, background): “Several lines of evidence indicate that miRNAs can also bind to other regions in the target mRNA [36].”