Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems

By N. Caplen, S. Parrish, F. Imani, Andrew Fire, R. Morgan. Proceedings of the National Academy of Sciences of the United States of America, 2001

1,110 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7241474d6551347cf4d8cc08787a38ecf09707dd

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Citations in the corpus

RNA interference targeting Fas protects mice from fulminant hepatitis (2003, background): “jpg" NDATA ITEM> ]> RNAi by synthetic siRNAs 21–23 nucleotides in length silences cellular and viral gene expression in mammalian cells in vitro”
The small RNA profile during Drosophila melanogaster development. (2003, background): “AtKurchatov sq. 2 least three types of small RNAs have been described:Moscow 123182 (1) Small interfering RNAs (siRNAs) (Caplen et al., 2001;Russia Elbashir et al., 2001a, 2001b), (2) microRNAs (miRNAs)2 Laboratory for RNA Molecular Biology (Lagos-Quintana et al., 2001; Lau et al., 2001; Lee andThe…”
Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy (2005, methodology): “Although others have used dsRNAs of up to 27 bp for RNAi studies in mammalian cell”
The next generation of rodent eradications: Innovative technologies and tools to improve species specificity and increase their feasibility on islands (2015, positive): “Inmiceandrats,RNAiselectivelyinhibitstargetgene expressionwithhighspeciﬁcityandefﬁcacy,andisrelativelyeasyto induce through injections (Caplen et al., 2001; Xue et al., 2012).”
Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. (2002, background): “This level of inhibition is similar to results from previous studies using luciferase reporter plasmid systems (23,24).”
On the art of identifying effective and specific siRNAs (2006, background): “It was noticed early that 20–25 nt siRNA duplexes carrying 2-nt 3′ overhangs could reach similar gene silencing efficiency in mammalian cell culture experiment”
ATP requirements and small interfering RNA structure in the RNA interference pathway. (2001, neutral): “RNAi (Hamilton and Baulcombe, 1999).”
On the role of RNA amplification in dsRNA-triggered gene silencing. (2001, neutral): “Whatever the mechanism by which synthesis of newpass the RNAi defect in rde-4 mutants (but not in rde-1 mutants) (Caplen et al., 2001; Parrish and Fire, 2001). antisense RNA is primed, the subsequent activity of DICER or another dsRNA-specific nuclease could func-When tested in the rrf-1 mutant…”
A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans (2004, background): “Injection of a synthetic 23-bp unc-22 siRNA with 2-nt 3′ overhangs caused up to 55 times more progeny of the injected eri-1(mg366) mutant (- ) animals than progeny of injected wild-type ( eri-1(p ) ) animals to show an unc-22 loss-of-function phenotype ( Table 1 ), indicating that ERI-1 functi”
Illuminating the silence: understanding the structure and function of small RNAs (2007, background): “Indeed, two groups reported that short dsRNA (∼21–23-nt) triggers could be transfected into mammalian cells to efficiently induce sequence-specific gene silencin”
Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells (2002, background): “Synthetic and endogenous siRNAs direct targeted mRNA degradatio”
Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. (2002, background): “, and development (Zou et al., 1998; Sharma et al., 2001; Deng and Lin, 2002; in D. melanogaster (Caplen et al., 2001; Elbashir et al., 2002).”
siRNAs: applications in functional genomics and potential as therapeutics (2004, background): “The elucidation of siRNA structure led to the discovery that siRNAs can effectively reduce gene expression in many mammalian cell types without triggering the IFN respons”