Localized Rac activation dynamics visualized in living cells.

By V. Kraynov, C. Chamberlain, G. Bokoch, M. Schwartz, Sarah Slabaugh, K. Hahn. Science, 2000

733 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/67288ab6e69bc4f765191d3cc90931e4cfadb9d9

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Citations in the corpus

Conserved microtubule–actin interactions in cell movement and morphogenesis (2003, background): “In support of this hypothesis, FRET-based visualization of Rac1 activity has revealed a gradient from the leading edg”
FAK–Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly (2004, background): “Because Rac is a key regulator of migration and active Rac localizes at the leading edge of motile cell”
Random versus directionally persistent cell migration (2009, background): “RAC1 is highly active at the leading edge during intrinsic migratio”
Cell migration: Rho GTPases lead the way. (2004, methodology): “Using the FLAIR technique (fluorescence activation indicator for Rho proteins), for example, a gradient of active Rac can be visualized in migrating fibroblasts with the highest concentrations at the leading edge (Kraynov et al., 2000).”
Molecular and Structural Basis for Redox Regulation of β-Actin (2007, background): “factors appears to target Rac1 to the membrane where, in migrating cells, actin reorganization also appears to be maximal, and causes its concomitant, spatially restricted activation.(66) Amplification of an H2O2 signal in vivo seems to occur through the integration of the Rac and phosphatidylinosit”
Rho GTPases and cell migration. (2001, background): “Indeed, a novel technique for detecting where Rac is active in living cells has revealed that active Rac is localized preferentially towards the front of migrating cells (Kraynov et al., 2000).”
The relationship between force and focal complex development (2002, background): “First, experimentally induced focal complexes were only observed on the cell lamella, where high GTPase Rac activity has been previously reported (Kraynov et al., 2000).”
Rho GTPases: biochemistry and biology. (2005, background): “GTP-bound active Rac accumulates at the front of migrating cells, and in chemotaxing neutrophils this is sustained through a positive feedback loop with PI 3-kinase and its lipid product PIP3 (Gardiner et al. 2002, Itoh et al. 2002, Kraynov et al. 2000).”
RHO GTPASES in neuronal morphogenesis (2000, background): “Indeed, while this manuscript was in review, the first imaging assay of Rac activity using fluorescence resonance energy transfer was reporte”
A Landscape of Driver Mutations in Melanoma (2012, methodology): “Plasmids pcDNA3-EGFP-RAC1 (wild-type, T17N, and Q61L) were obtained from Addgene (plasmids 13719, 13720, and 13721) courtesy of Klaus Hahn (Kraynov et al., 2000). pcDNA3-EGFP-RAC1(P29S) was generated by using QuikChange Lightning Site-Directed Mutagenesis (Stratagene, Santa Clara, CA) according to t”
Riding the DUBway: regulation of protein trafficking by deubiquitylating enzymes (2006, unknown): “Active Rac1, for instance, concentrates within ruffl ing lamellae, suggesting spatial coordination of Rac's cytoskeletal and prooxidant effects (Kraynov et al., 2000).”
Adhesion-dependent cell mechanosensitivity. (2003, background): “…visualization of active Rho has not been reported yet, there are some interesting data concerning the distribution of activated Rac and Cdc42 based on various modifications of the fluorescence resonance energy transfer (FRET) technique (Gardiner et al. 2002, Itoh et al. 2002, Kraynov et al. 2000).”
Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI (2002, background): “While studying Rac activation and localization with a FRET assa”