An evaluation of methods for extraction and quantification of protein from marine macro- and microalgae

By E. Barbarino, S. O. Lourenço. Journal of Applied Phycology, 2005

382 citations recorded by the source corpus.

Subfields: Aquatic Science, Global and Planetary Change, Oceanography

View Paper: https://www.semanticscholar.org/paper/64788e38cb2f6443812262b94fa2126c2b666c67

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aquatic Science

1. M. Hayes
2. S. Bleakley
3. C. Vaca-Garcia
4. P. Pontalier
5. C. Safi
6. K. Bischof
7. L. Hofmann
8. Sandra C. Straub

In Global and Planetary Change

1. M. Hayes
2. S. Bleakley
3. David Raubenheimer
4. Z. Finkel
5. C. Vaca-Garcia
6. P. Pontalier
7. C. Safi
8. J. Raven

In Oceanography

1. J. Raven
2. Z. Finkel
3. John Beardall
4. M. Hayes
5. Antonietta Quigg
6. L. Hofmann
7. K. Bischof
8. S. Bleakley

Citations in the corpus

• Influence of microalgae cell wall characteristics on protein extractability and determination of nitrogen-to-protein conversion factors (2012, background): “In addition, the high values of hydro-soluble proteins extracted especially for P. cruentum and A. platensis could be explained by the possible presence of peptides and free amino acids because no precipitation was carried out (Nguyen and Harvey 1994; Barbarino and Lourenço 2005).”
• IS THE GROWTH RATE HYPOTHESIS APPLICABLE TO MICROALGAE? 1 (2010, methodology): “Neither assay yields protein values that correspond to values computed more directly and reliably from the amino-acid composition of extracted proteins (Crossman et al. 2000, Barbarino and Lourenço 2005).”
• Algal Proteins: Extraction, Application, and Challenges Concerning Production (2017, positive): “Physical Processes Barbarino and Lourenço (2005) reported that physical grinding with the use of a Potter homogeniser significantly increased protein extraction yield from Porphyra acanthophora var. acanthophora , Sargassum vulgare , and Ulva fasciata following immersion in ultra-pure water (Table 1)”
• Amino acid composition, protein content, and nitrogen-to-protein conversion factors of 21 seaweed species from Norwegian waters (2017, background): “Protein extraction procedures are not optimal for seaweeds, and chemical features of the algal material often interfere with both protein extraction and the colorimetric assays, leading to a biased and inaccurate quantification of the protein content (Fleurence 1999a, Barbarino and Lourenço 2005).”
• Protein measurements of microalgal and cyanobacterial biomass. (2010, background): “Pretreatments typically involve disrupting the cells by physical or chemical means (Barbarino and Lourenço, 2005; Guerlava et al., 1998).”
• In the search of new functional food ingredients from algae (2008, background): “According to Barbarino and Lourenco (2005), proteins of S.”
• Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa (2016, positive): “Microalgae proteins have a great potential to be an alternative protein source since they contain all essential amino acids (Becker 2007; Barbarino and Lourenço 2005; Lourenco et al.”
• Bioactive compounds in seaweed: functional food applications and legislation (2011, background): “(2007); d Jensen and Haug (1956); e Bartsch et al. (2008); f Zubia et al.”
• Competition between calcifying and noncalcifying temperate marine macroalgae under elevated CO2 levels (2012, methodology): “Methods for measuring phycoerythrin and phycocyanin were taken from Beer & Eshel (1985), protein precipitation was conducted according to Barbarino & Lourenço (2005), and protein concentration was determined by the Bradford method (Bradford 1976).”
• Nutritional value of selected macroalgae (2011, result): “In general, the crude protein content recorded for the studied red and brown seaweeds was similar to the one reported in other studies (e.g., Fleurence 1999; Rupérez and Saura-Calixto 2001; Burtin 2003; McDermid and Stuercke 2003; Barbarino and Lourenço 2005; Marinho-Soriano et al. 2006; Dawczynski ”
• Nutritional ecology of marine herbivorous fishes: ten years on (2009, background): “This strategy is perhaps driven by the distinctive amino acid composition of algal proteins (Fleurence 1999; Barbarino & Lourenço 2005).”
• Morphology, composition, production, processing and applications of Chlorella vulgaris: A review (2014, methodology): “The Lowry assay is more acceptable than the Bradford assay because the latter does not react with all the amino acids present in the extract, thus giving lower protein concentrations [92].”