Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa
By A. Waghmare, M. Salve, J. LeBlanc, S. Arya. Bioresources and Bioprocessing, 2016
210 citations recorded by the source corpus.
BackgroundMany methods are available for the concentration of proteins; however, most are not easily scalable due to costs, the need of specialized instruments and skilled workers or are very time-consuming. Three-phase partitioning (TPP) is a separation technique that has gained a lot of interest due to its rapid, simple and scalable use for concentration, isolation and decontamination of proteins from crude samples with high recovery yields. In the present work, the effect of various parameters of TPP was evaluated to optimize the concentration of proteins from Chlorella pyrenoidosa (CP), is green algae that increasingly being used as food supplements because of its positive impacts on human health.ResultsChlorella pyrenoidosa was cultivated in a closed system under controlled conditions. After reaching maximum growth, the microalgae was harvested, dried and powdered. Afterwards, TPP of CP cell lysate was done to concentrate protein content. To maximize protein concentration, various parameters were optimized such as solvent (t-butanol), ammonium sulphate concentration (40 % w/v), solid load (0.75 g/20 mL), pH (6), incubation time (20 min), slurry to butanol ratio (1:1.5) and enzymatic treatment (combination of Stargen™ and Carezyme™). Also, total starch, cellulose and carbohydrate content before and after the enzymatic treatment were determined to comprehend the impact of enzymatic treatment on protein concentration. Using these optimized parameters, 78.1 % w/w protein concentration was obtained in middle protein concentrate phase. This protein concentrate was characterizedfor proximate composition, colour analysis, water holding capacity, oil-holding capacity, foaming capacity, foam stability, amino acid composition, protein quality and thermal properties.ConclusionVarious process parameters of TPP influence the protein concentration of middle protein concentrate phase. Enzymatically treated biomass also enhanced protein concentration in middle protein concentrate phase. Characterization of protein concentrate revealed the presence high-quality protein. Therefore, it is possible to implement TPP at an industrial scale for protein concentration.Graphical abstractConcentration of Chlorella pyrenoidosa proteins using three phase partitioning method
Subfields: Filtration and Separation, Renewable Energy, Sustainability and the Environment
View Paper: https://www.semanticscholar.org/paper/2bde67685ad96b63a273d6dc06feaee5dcc07462
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
Citations in the corpus
- Single Cell Protein—State-of-the-Art, Industrial Landscape and Patents 2001–2016 (2017, background): “…(Spirulina maxima) 60–71 De Oliveira et al., 1999 Arthospira platensis (Spirulina platensis) 46–63 Rafiqul et al., 2005 Chlorella pyrenoidosa 45 Waghmare et al., 2016 Chlorella sorokiana 46–65 Safafar et al., 2016 Chlorella spp. 62–68 Liu et al., 2013 Chlorella vulgaris 42–55 Li et al.,…”