Active Wnt proteins are secreted on exosomes

By J. Gross, Varun Chaudhary, Kerstin Bartscherer, Michael Boutros. Nature Cell Biology, 2012

943 citations recorded by the source corpus.

Subfields: Immunology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/63f1fc897a8fb497dae4bb9c5c9543ffc12e2b3f

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In Immunology

1. C. Théry
2. G. Raposo
3. Marina Colombo
4. W. Stoorvogel
5. R. Nusse
6. Hans Clevers
7. T. Whiteside

In Molecular Biology

1. C. Théry
2. T. Whiteside
3. G. Raposo
4. Marina Colombo
5. Hans Clevers
6. A. Llorente
7. R. Nusse
8. J. Kowal

Citations in the corpus

• Mechanisms of intercellular Wnt transport (2019, background): “Furthermore, in vivo staining of the Drosophila wing disc revealed colocalisation of Wg and the exosomal marker CD63-GFP in both intracellular MVBs and the extracellular space, although this was only a fraction of the total Wg staining (Gross et al., 2012).”
• Biological properties of extracellular vesicles and their physiological functions (2015, background): “Shuttle of functional Wnt proteins to MVBs required ESCRT and was mediated by their cargo receptor Evi Ykt6 protein, which regulates early/recycling endosomes (549).”
• Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function (2015, background): “They can also be identified by the presence of proteins common to most exosomes, such as the tetraspanin proteins CD63, CD9, and CD81 [1–3].”
• Current knowledge on exosome biogenesis and release (2017, background): “Later, Hrs depletion in HEK293 cells was shown to reduce exosomal Wnt3A and Evi secretion [33].”
• Biogenesis and secretion of exosomes. (2014, positive): “Four independent studies have shown a requirement for the ESCRT-0 member HRS in exosome secretion, demonstrated by a decrease in exosome secretion upon HRS inhibition in various cell types: HeLa cells [32], mouse DCs (but only after incubation with a antigen)[33], HEK293 [34] and head and neck squam”
• Tumor-derived exosomes and their role in cancer progression (2016, background): “The ability of tumor-derived exosomes produced by mouse melanoma cells to educate and transform the bone marrow environment into a melanoma-promoting milieu has been elegantly demonstrated by Peinado et al [32] and exosome-mediated alterations of the bone marrow have been shown to interfere with the”
• An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control (2014, background): “Some Wnt proteins may be incorporated into secretory vesicles (3), in which Wls continues to bind Wnt proteins (4) as a chaperone (Fig.”
• Structural basis of WLS/Evi-mediated Wnt transport and secretion (2020, background): “Wnt and WLS can transfer together across synapses on lipid vesicles such as exosomes, and (iii) WLS and Wnt are transferred together to the receiving cell membrane in the Drosophila brain (Gross et al., 2012; Koles et al., 2012; Korkut et al., 2009).”
• Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration. (2012, background): “These include lateral diffusion by association with heparan sulfate proteoglycans (HSPGs), solubilization by high-density lipoproteins, carrier proteins (Coudreuse and Korswagen, 2007; Willert and Nusse, 2012), and exosomes (Gross et al., 2012).”
• Wnt/ b -Catenin Signaling, Disease, and Emerging Therapeutic Modalities (2017, background): “More likely, Wnt proteins are incorporated into secretory vesicles or exosomes (Gross et al., 2012; Korkut et al., 2009; McGough and Vincent, 2016; Saha et al., 2016; Gross et al., 2012).”